Recombinant Full Length Human ATP5B Protein, GST-tagged

• Cat.No.: ATP5B-1339HF
• Product Overview: Human ATP5B full-length ORF ( NP_001677.2, 1 a.a. - 529 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 83 kDa
• Protein Length: 529 amino acids
• AA Sequence: MLGFVGRVAA APASGALRRL TPSASLPPAQ LLLRAAPTAV HPVRDYAAQT SPSPKAGAAT GRIVAVIGAV VDVQFDEGLP PILNALEVQG RETRLVLEVA QHLGESTVRT IAMDGTEGLV RGQKVLDSGA PIKIPVGPET LGRIMNVIGE PIDERGPIKT KQFAPIHAEA PEFMEMSVEQ EILVTGIKVV DLLAPYAKGG KIGLFGGAGV GKTVLIMELI NNVAKAHGGY SVFAGVGERT REGNDLYHEM IESGVINLKD ATSKVALVYG QMNEPPGARA RVALTGLTVA EYFRDQEGQD VLLFIDNIFR FTQAGSEVSA LLGRIPSAVG YQPTLATDMG TMQERITTTK KGSITSVQAI YVPADDLTDP APATTFAHLD ATTVLSRAIA ELGIYPAVDP LDSTSRIMDP NIVGSEHYDV ARGVQKILQD YKSLQDIIAI LGMDELSEED KLTVSRARKI QRFLSQPFQV AEVFTGHMGK LVPLKETIKG FQQILAGEYD HLPEQAFYMV GPIEEAVAKA DKLAEEHSS
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ATP5B ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide [ Homo sapiens ]
• Official Symbol: ATP5B
• Synonyms: ATP5B; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; ATPSB; ATP synthase subunit beta, mitochondrial; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthetase, beta subunit; ATPMB; MGC5231
• Gene ID: 506
• mRNA Refseq: NM_001686
• Protein Refseq: NP_001677
• MIM: 102910
• UniProt ID: P06576

Reviews

05/22/2020

The outstanding quality of proteins lies in their strong biological activity and their high purity during production, which is really rare.

04/25/2020

ATP5B has a very short half-life and shows a very high clearance rate, which makes its biological activity more stable.

04/07/2019

Because the production process is efficient and environmentally friendly, recombinant proteins can be produced on a large scale without negative environmental impacts.

Q&As

What is the frequency of ATP5B mutations?

ATP5B gene mutation frequency may be different in different diseases and populations, which needs further study.

How about the link between ATP5B protein and neurological diseases?

It plays an important role in the nervous system and is associated with certain nervous system diseases such as Parkinson's disease and cerebral ischemia.

How are ATP5B mutations associated with disease?

This gene mutations may lead to abnormal protein structure or function, which in turn affects the physiological function of the protein and is associated with specific diseases.

What are the regulatory mechanisms of ATP5B?

ATP5B expression and function are affected by a variety of regulatory mechanisms, including regulation of transcription factors and epigenetic modifications.

How to evaluate the effect of ATP5B gene mutation on disease?

Assessment of the effect of ATP5B mutations on disease often requires a comprehensive analysis of gene sequencing, protein function studies, and clinical and epidemiological studies.

In which tissues or organs is ATP5B highly expressed?

It is highly expressed in multiple tissues and organs, especially metabolically active tissues such as heart, liver, and brain.