Recombinant Full Length Human SLC4A1 Protein, C-Flag-tagged

• Cat.No.: SLC4A1-89HFL
• Product Overview: Recombinant Full Length Human SLC4A1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) results from the heterozygous presence of a deletion in the encoded protein and is common in areas where Plasmodium falciparum malaria is endemic. One null mutation in this gene is known, resulting in very severe anemia and nephrocalcinosis.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 101.6 kDa
• AA Sequence: MEELQDDYEDMMEENLEQEEYEDPD IHESQMEEPAAHDTEATATDYHTTS HPGTHKVYVELQELVMDEKN QELRWMEAARWVQLEENLGENGAWG RPHLSHLTFWSLLELRRVFTKGTVL LDLQETSLAGVANQLLDRFI FEDQIRPQDREELLRALLLKHSHAG ELEALGGVKPAVLTRSGDPSQPLLP QHSSLETQLFCEQGDGGTEG HSPSGILEKIPPDSEATLVLVGRAD FLEQPVLGFVRLQEAAELEAVELPV PIRFLFVLLGPEAPHIDYTQ LGRAAATLMSERVFRIDAYMAQSRG ELLHSLEGFLDCSLVLPPTDAPSEQ ALLSLVPVQRELLRRRYQSS PAKPDSSFYKGLDLNGGPDDPLQQT GQLFGGLVRDIRRRYPYYLSDITDA FSPQVLAAVIFIYFAALSPA ITFGGLLGEKTRNQMGVSELLISTA VQGILFALLGAQPLLVVGFSGPLLV FEEAFFSFCETNGLEYIVGR VWIGFWLILLVVLVVAFEGSFLVRF ISRYTQEIFSFLISLIFIYETFSKL IKIFQDHPLQKTYNYNVLMV PKPQGPLPNTALLSLVLMAGTFFFA MMLRKFKNSSYFPGKLRRVIGDFGV PISILIMVLVDFFIQDTYTQ KLSVPDGFKVSNSSARGWVIHPLGL RSEFPIWMMFASALPALLVFILIFL ESQITTLIVSKPERKMVKGS GFHLDLLLVVGMGGVAALFGMPWLS ATTVRSVTHANALTVMGKASTPGAA AQIQEVKEQRISGLLVAVLV GLSILMEPILSRIPLAVLFGIFLYM GVTSLSGIQLFDRILLLFKPPKYHP DVPYVKRVKTWRMHLFTGIQ IICLAVLWVVKSTPASLALPFVLIL TVPLRRVLLPLIFRNVELQCLDADD AKATFDEEEGRDEYDEVAMP
  VTRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane

Gene Information

• Gene Name: SLC4A1 solute carrier family 4 member 1 (Diego blood group) [ Homo sapiens (human) ]
• Official Symbol: SLC4A1
• Synonyms: DI; FR; SW; WD; WR; AE1; CHC; SAO; WD1; BND3; EPB3; SPH4; CD233; EMPB3; RTA1A
• Gene ID: 6521
• mRNA Refseq: NM_000342.4
• Protein Refseq: NP_000333.1
• MIM: 109270
• UniProt ID: P02730

Reviews

05/18/2022

Timely delivery, so I got the desired experimental results in the shortest possible time.

05/10/2021

Friendly customer service and detailed answers to all the questions I asked!

12/30/2020

The technicians were very professional and suggested improvements to my experimental program, which allowed the experiment to go smoothly.

Q&As

Where is SLC4A1 mainly expressed?

SLC4A1 is expressed on the erythrocyte membrane and the basement membrane surface of renal type A intercalary cells.

What are the effects of mutations in the SLC4A1?

The reduced amount of SLC4A1 protein following the SLC4A1 mutation makes the erythrocyte membrane less stable and prone to detachment, and the resulting reduction in surface area/volume changes the shape of the erythrocyte from a biconcave disc shape to a spherical shape.

What biological function does SLC4A1 have?

The main function of SLC4A1 is to carry out HCO3-/Cl- transmembrane transport.

What diseases has SLC4A1 been closely associated with the study of?

SLC4A1 has been closely associated with studies of diseases such as distal renal tubular acidosis and hereditary spherocytosis.