Recombinant Full Length Schizosaccharomyces Pombe Alpha-1,3/1,6-Mannosyltransferase Alg2(Alg2) Protein, His-Tagged

• Cat.No.: RFL25299SF
• Product Overview: Recombinant Full Length Schizosaccharomyces pombe Alpha-1,3/1,6-mannosyltransferase alg2(alg2) Protein (Q96WW6) (1-511aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
• Tag: His
• Form: Lyophilized powder
• Protein Length: Full Length (1-511)
• AA Sequence: MSQENSVHRSSTKKTPIKIAFIHPD LGIGGAERLVVDAAVGLQSLGKEVV VFTSHCDKKH CFEEIRDGTIKVKVYGDWLPSSIFG RLSIFCSSLRQVYLTMILLTNYMHF DAIIVDQLST CVPFLLLASQMILFYCHFPDKYLAK RGGILKKLYRIPFDTVEAESVRLAD RIVVNSKFTA SVFKKAFPKIRKPLRIVHPCVDIEA ASKPLEFQLPEKILYLRYSQRKLLI SVNRFERKKD IRLAIDAFSALRDLSANRFPEYLLL VAGGYDIRVSENRRYLKELQEFCEQ KDLSYTTVKD NWDNITVAPSTNVLFLLSVPSKVRD ALISSSRILLYTPENEHFGIVPLEA MLRKVPVLAQ TNGGPLETVIDGKNGWLRPRDAKIW GNVIYEATTSTTYDTAAMGEAGSEW VKNEFSTDAM ARKFESEIMSGIRSITPEKRLMRRV NGLLAVFVLFMLFWGTCIIAATVPF AIIKLYFAQT YSSVKLGFMLGTCIVSVSFLTFTVY AKLTNL
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: alg2
• Synonyms: alg2; pi010; SPBC11B10.01; SPBC32H8.14; Alpha-1,3/1,6-mannosyltransferase alg2; Asparagine-linked glycosylation protein 2; GDP-Man:Man(1GlcNAc(2-PP-Dol alpha-1,3-mannosyltransferase; GDP-Man:Man(1GlcNAc(2-PP-dolichol mannosyltransferase; GDP-Man:Man(2GlcN
• UniProt ID: Q96WW6

Gene Information

• Gene Name: alg2
• Synonyms: alg2; pi010; SPBC11B10.01; SPBC32H8.14; Alpha-1,3/1,6-mannosyltransferase alg2; Asparagine-linked glycosylation protein 2; GDP-Man:Man(1GlcNAc(2-PP-Dol alpha-1,3-mannosyltransferase; GDP-Man:Man(1GlcNAc(2-PP-dolichol mannosyltransferase; GDP-Man:Man(2GlcN
• UniProt ID: Q96WW6

Reviews

07/20/2022

In immunological experiments, ALG2 was found to have good antigenicity and could induce target immune responses.

02/01/2021

The high solubility of ALG2 makes it easy to prepare the desired concentration.

09/03/2020

The high catalytic efficiency of ALG2 was found in enzymatic experiments.

11/23/2019

It was observed that ALG2 had better crystallinity, which was beneficial for crystallographic studies.

Q&As

Does ALG2 mutation have an adverse effect on embryonic development?

This mutations can have adverse effects on embryonic development and may therefore be associated with a number of fetal diseases.

What biological processes does ALG2 participate in?

ALG2 is involved in several biological processes including protein glycosylation, ER and Golgi body sugar metabolism.

Which cell signaling molecules are involved in ALG2?

ALG2 regulates the expression of glycosylase and the balance of glycosylation reaction by interacting with other glycosylated enzymes such as ALG3 and ALG1.

What are the effects of ALG2 inhibitors?

A number of inhibitors have been developed that target ALG2 and inhibit its glycosylation activity, thereby interfering with its function in cellular processes.

What are the action mechanisms of ALG2 ?

The catalyzes the sugar chain transfer reaction between UDP-glucose and substrate proteins, synthesizing sugar chains onto proteins.

Does ALG2 participate in mitochondrial glucose metabolism?

No studies have confirmed whether ALG2 is involved in mitochondrial glucose metabolism, and more studies are needed to prove it.

What non-cellular autophagy pathways are involved in ALG2?

Although there are currently no studies demonstrating the function of ALG2 in non-cellular autophagy pathways, there are indications that it may be involved in the TCA cycle and gluconeogenic pathways.

What are the molecular biological characteristics of ALG2?

ALG2 plays an important role in protein glycosylation pathway, participating in the synthesis and modification of sugar chains.

What effect does overexpression or knockout of ALG2 have on cells?

Overexpression or knockout of ALG2 can negatively affect cellular glycosylation and the stability of ER and Golgi bodies, but the specific effects need to be further explored.

What is the sugar chain selectivity of ALG2?

This protein selectively catalyzes the synthesis and modification of sugar chains and plays an important role in the binding selectivity of glycosylase family members to substrate proteins.

How is ALG2 distributed in mammalian cells?

ALG2 is mainly distributed in the endoplasmic reticulum and Golgi apparatus in mammalian cells.

In which cancers does ALG2 play a role?

At present, there is no direct evidence that ALG2 is related to the occurrence and development of cancer, but glycosylation plays an important role in metabolism and disease development, so it may be relevant to related diseases.

In what neurological diseases does ALG2 play a role?

There is currently no direct evidence that ALG2 is associated with neurological diseases, but glycosylation plays a key role in nervous system development and function, so there may be a correlation with neurological diseases.

What is the gene structure and regulation of ALG2?

This gene contains 10 exons, and its expression is influenced by multiple regulatory elements and signaling pathways.

Which place of ALG2 in cellular processes?

ALG2 is mainly distributed in the endoplasmic reticulum (ER) and the Golgi apparatus.

Which diseases do ALG2 mutations cause?

At present, there is no direct evidence that ALG2 mutations are directly related to some diseases, but glycosylation plays an important role in metabolism and disease development, so it may be related to related diseases.

What are the interacting proteins of ALG2?

The protein interacts with members of the glycosylase family such as ALG3, ALG1 and other related proteins to jointly regulate the glycosylation process of proteins.

In what metabolic diseases does ALG2 play a role?

There is no direct evidence that ALG2 is involved in the occurrence and development of metabolic diseases, but it may be associated with related metabolic diseases.