Recombinant Full Length Yarrowia Lipolytica Chitobiosyldiphosphodolichol Beta-Mannosyltransferase(Alg1) Protein, His-Tagged

• Cat.No.: RFL28465YF
• Product Overview: Recombinant Full Length Yarrowia lipolytica Chitobiosyldiphosphodolichol beta-mannosyltransferase(ALG1) Protein (Q6C3K2) (1-463aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
• Tag: His
• Form: Lyophilized powder
• Protein Length: Full Length (1-463)
• AA Sequence: MKAWHWSVTLVVIYLAIPVILYLLT RKDDRKPLSDIRKRKRTIVLVLGDL GRSPRMLYHA RSLARSGHKVDLCGYDGAKPFDEIL NNDLIKIHHIPLILNTRKLPFVVFG ILKVIRQHWL LISLLYKLRGADYLLVQNPPSIPTL GVVRFYNLFLSTRTKVVLDWHNFGY TILALKLPET HPMVKFAKFYEGFFGGRAFVHLCVT VLMGQAMRKTFGMSGRRIVPLHDRP AFHFKPLSES EKLDVLRDFKETLYDDMTADHKIIV SSTSYTPDENFNILLDALALYDESK LDLPPLRVII TGKGPMMPEFLAKVEKLQLKRVSIR TAWLEFADYPRILGAAHLGVSLHES SSGYDLPMKV VDMFGCGIPVVSVDYAALSELVKTN TNGVAVKGHVEMGNTFMSLFSNRGK LDNIKRGAMI ESRNTWDQTWVKTVGPLFDIGEYVQ QRPDEDYDFSSSSSDDDH
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: ALG1
• Synonyms: ALG1; YALI0E34133g; Chitobiosyldiphosphodolichol beta-mannosyltransferase; Asparagine-linked glycosylation protein 1; Beta-1,4-mannosyltransferase; GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase; GDP-mannose-dolichol diphosphochitobiose mannosyltransfera
• UniProt ID: Q6C3K2

Gene Information

• Gene Name: ALG1
• Synonyms: ALG1; YALI0E34133g; Chitobiosyldiphosphodolichol beta-mannosyltransferase; Asparagine-linked glycosylation protein 1; Beta-1,4-mannosyltransferase; GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase; GDP-mannose-dolichol diphosphochitobiose mannosyltransfera
• UniProt ID: Q6C3K2

Reviews

02/24/2022

The protein specificity is high and the misdiagnosis rate is low.

12/22/2020

The most suitable detection method is ELISA.

12/15/2020

High sensitivity in diagnosis.

04/13/2020

Detection limit is low, low content can also be successfully detected。

Q&As

What is the ALG1 test fee?

ALG1 testing costs vary by hospital and region and may fluctuate within a range.

Does ALG1 detection require special preparation and precautions?

ALG1 testing does not require special preparation before it is performed, but if a blood sample needs to be collected, the blood collection process needs to be completed under the guidance of a doctor.

What groups of people are more susceptible to ALG1 defects?

ALG1 deficiency is a genetic disorder, so it may affect members of its family.

How is ALG1 defect caused?

ALG1 defects are usually caused by mutations, loss or deletion of genes, which in turn affect the metabolism of protein glycosylation.

Does ALG1 defect affect fertility?

ALG1 defects may affect fertility and can cause problems such as genetic disorders.

What is the role of ALG1 in the human body?

ALG1 is an important gene involved in protein glycosylation in human body, and plays an important role in maintaining the normal function of human body.

Is ALG1 testing routine?

Testing is not a routine procedure and is usually done on the advice of a doctor.

What factors interfere with ALG1 detection?

Detection can be interfered with by a number of factors, such as clinical symptoms, age, gender, diet, and so on.

Is there a familial risk of ALG1 deficiency?

Deficiency has a familial genetic risk, and the risk of developing the disease depends on the family genetic history.

How to interpret the test results of ALG1?

ALG1 test results need to be interpreted and analyzed by a professional.

Does the ALG1 test require a professional?

ALG1 testing is performed by a professional clinical geneticist and laboratory technician.

What diseases is the ALG1 test mainly used to detect?

ALG1 detection is mainly used to detect CDG1K and other related diseases.

Can ALG1 defects be inherited?

ALG1 deficiency is a genetic disorder that can be passed on to future generations through genes.

What Clinical application ALG1 tested for?

This protein can be tested in the form of blood or oral swabs.

Does ALG1 test require fasting?

Testing does not require fasting.

What diseases can ALG1 defect cause?

Deficiency of ALG1 may cause a rare glycoprotein Disorder called Glycosylation 1k syndrome (CDG1K), which includes mental retardation, motor degeneration, and muscle relaxation.

What are the symptoms of ALG1 defect?

Deficiency may cause mental retardation, motor function degradation, fluid on the head, eye problems and other symptoms.

How is ALG1 defect treated?

Deficiency can be treated with targeted treatment for its clinical manifestations, such as symptom relief, rehabilitation, etc.

Can ALG1 test protect against disease?

Testing may help in the early detection of related diseases, so that targeted prevention and treatment measures can be taken, but often cannot prevent all related diseases.