Recombinant Human ABHD11 293 Cell Lysate

• Cat.No.: ABHD11-9140HCL

Specification

• Description: Antigen standard for abhydrolase domain containing 11 (ABHD11), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ABHD11 abhydrolase domain containing 11 [ Homo sapiens ]
• Official Symbol: ABHD11
• Synonyms: ABHD11; abhydrolase domain containing 11; WBSCR21, Williams Beuren syndrome chromosome region 21; abhydrolase domain-containing protein 11; PP1226; Williams Beuren syndrome chromosome region 21; williams-Beuren syndrome chromosomal region 21 protein; WBSCR21
• Gene ID: 83451
• mRNA Refseq: NM_001145364
• Protein Refseq: NP_001138836
• UniProt ID: Q8NFV4
• Chromosome Location: 7q11.23
• Function: hydrolase activity; molecular_function

Reviews

07/10/2022

Great work on ELISA with recommended dilution !

06/07/2022

Excellent quality products!

11/06/2021

We are satisfied with the WB results of ABHD11 product.

03/15/2021

After-sales questions were answered in a timely manner. Good!

Q&As

What kind of disease is ABHD a biomarker?

ABHD11 is a potential biomarker of lung adenocarcinoma, and is linked to breast cancer malignancy.

What is the influence of ABHD11 for 2-oxoglutarate in metabolic pathways?

ABHD11 loss or inhibition drives a rapid increase in 2-OG levels by impairing lipoylation of the 2-OG dehydrogenase complex (OGDHc)—the rate limiting step for mitochondrial 2-OG metabolism.

What disease caused by the ABHD11 abnormal?

ABHD11 is one of several genes deleted in Williams–Beuren syndrome, a developmental disorder associated with haploinsufficiency of multiple genes.

What fact suggest that ABHD11 was involved in the maintenance of embryonic stem cell identity?

The results of bioinformatic analysis demonstrated that ABHD11 and ABHD10 were downregulated, while most other ABHD family genes, such as Abhd 1, 3, 5, 6, and 7 were upregulated in OCT4-negative cells.

What is the consequences if knockout ABHD11 gene?

ABHD11 knockout disturbed GSK3β/β-Catenin and ERK signaling transduction; ABHD11 knockout led to the misexpression of key metabolic enzymes related to lipid synthesis, glycolysis, and amino acid metabolism.

What disease often use the ABHD11 as prognostic marker?

The ABHD11 often as a prognostic marker of renal cancer.

Where does the ABHD11 protein express in human body?

Kidney - Transmembrane transport (mainly)

What is the results if the ABHD11 overexpress in yeast?

In yeast, the overexpression of ABHD11 or its yeast homolog causes a reduction in the levels of non-polar lipids.

List at least three cellular components of the ABHD11 protein?

1. plasma membrane, 2. endosome, 3. mitochondrion

What results we got when a study conlcuded that qRT-PCR to show that the ABHD11-AS1 expression level was higher in ovarian cancer tissues than in normal ovarian tissue?

This results suggested that the ABHD11 gene promote cancer cell proliferation, metastasis, and invasion, and inhibit cancer cell apoptosis.