Recombinant Human ADAMTS5 293 Cell Lysate

• Cat.No.: ADAMTS5-9028HCL

Specification

• Description: Antigen standard for ADAM metallopeptidase with thrombospondin type 1 motif, 5 (ADAMTS5) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADAMTS5 ADAM metallopeptidase with thrombospondin type 1 motif, 5 [ Homo sapiens ]
• Official Symbol: ADAMTS5
• Synonyms: ADAMTS5; ADAM metallopeptidase with thrombospondin type 1 motif, 5; a disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase 2); A disintegrin and metalloproteinase with thrombospondin motifs 5; ADAMTS11; ADMP 2; aggrecanase 2; aggrecanase-2; a disintegrin and metalloproteinase with thrombospondin motifs 11; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2); ADMP-2; ADAM-TS5; ADAMTS-5; ADAM-TS 5; ADAMTS-11; ADAM-TS 11
• Gene ID: 11096
• mRNA Refseq: NM_007038
• Protein Refseq: NP_008969
• MIM: 605007
• UniProt ID: Q9UNA0
• Chromosome Location: 21q21.3
• Pathway: Endochondral Ossification, organism-specific biosystem
• Function: integrin binding; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding

Reviews

06/24/2020

The staff was very nice, communicated with me positively and solved a lot of technical problems.

06/19/2020

The ADAMTS5 proteins purchased from your company is suitable for ELISA.

06/17/2020

Fast and timely shipment helped me to get satisfactory experimental results in short time.

Q&As

Do you offer ADAMTS5 fused with other tags?

We can provide you with the most suitable labels according to your experimental needs.

ADAMTS5 is used in studies of which diseases?

ADAMTS5 is used in the study of oral squamous cell carcinoma, gastric cancer, and many other cancers.

How does ADAMTS5 relate to Aggrecan?

ADAMTS5 can cut Aggrecan.

What is the structure domain of ADAMTS5?

DAMTS5 is composed of a signal peptide, a pro-domain, a catalytic metalloproteinase domain, a disintegrin-like domain, and other C-terminal ancillary domains (a central thrombospondin type 1 sequence repeat (central TSR) motif, a cysteine-rich domain, a spacer region, and an additional TSR motif).

What biological processes is ADAMTS5 involved in?

ADAMTS5 is involved in the degradation of polyproteoglycans.