Active Recombinant Human ADAMTS5 Protein, His-tagged

• Cat.No.: ADAMTS5-144H

Specification

• Description: Synthetic human ADAMTS5 DNA sequence, amino acid residues 626-930 with N-terminal signal peptide and C-terminal 6His-Tag expressed in the CHO cell expression system. This protein binds to murine monoclonal antibodies of defined epitope specificity.
  ADAMST5: ADAMST5 defined as a disintegrin-metalloproteinase with thrombospondin motif 5, also known as aggrecanase-2 and ADAMST511, is a member of the family of secreted zinc proteases with a multi-domain structure. The protein precursor consists of signal peptide , a pro-catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, spacer and a variable number of TS type 1 motifs. ADAMST5 is an active protease effectively cleaving alpha-2 macroglobulin, aggrecan and brevican, and is inhibited by TIMP-3. The purified rhADAMSt5 starts at the N-terminus of the catalytic domain and ends at the C-terminus of the TSP-1 domain. The amino acid sequence of rhADAMST5 has 95-98% homology with canine, bovine and murine proteins.
• Source: CHO cell
• Species: Human
• Tag: His
• Form: Frozen Liquid
• Bio-activity: Measured by its ability to cleave human Aggrecan (native and recombinant). Substrate and enzyme in a molar ratio of 10:1 (10ug subtrate, 1ug enzyme) in buffer (50mM Tris, pH 7.5, 10mM CaCl2, 0.15M NaCl, 0.05% Brij-35 incubated for 2-4 hours at 37C. The degradation products were analyzed by SDS-PAGE Western blots using epitope-specific monoclonal antibodies and molecular mass determination of aggrecan degradation products.
• Molecular Mass: 54 kD
• Purity: > 95 % by SDS-PAGE
• Applications: Aggrecanase Assays;
  Drug Screening.
• Notes: Recommended concentrations for use are approximate values. A substrate/enzyme assays sould be performed to determine the optimal ratios for specific applications.
• Stability: This protein is stable for upto 12 months at -75 centigrade. Aliquote protein for long term use and freeze at -75 centigrade. Avoid repeated freezing –thawing of the protein. Endotoxin content of the protein: <1000EU/mg.
• Storage: Store at -75 centigrade.
• Dilutions: 20mM Na-Ac, 0.6M NaCl, 0.01M CaCll2, 0.035% Brij-35

Reviews

06/24/2020

The staff was very nice, communicated with me positively and solved a lot of technical problems.

06/19/2020

The ADAMTS5 proteins purchased from your company is suitable for ELISA.

06/17/2020

Fast and timely shipment helped me to get satisfactory experimental results in short time.

Q&As

Do you offer ADAMTS5 fused with other tags?

We can provide you with the most suitable labels according to your experimental needs.

ADAMTS5 is used in studies of which diseases?

ADAMTS5 is used in the study of oral squamous cell carcinoma, gastric cancer, and many other cancers.

How does ADAMTS5 relate to Aggrecan?

ADAMTS5 can cut Aggrecan.

What is the structure domain of ADAMTS5?

DAMTS5 is composed of a signal peptide, a pro-domain, a catalytic metalloproteinase domain, a disintegrin-like domain, and other C-terminal ancillary domains (a central thrombospondin type 1 sequence repeat (central TSR) motif, a cysteine-rich domain, a spacer region, and an additional TSR motif).

What biological processes is ADAMTS5 involved in?

ADAMTS5 is involved in the degradation of polyproteoglycans.