Recombinant Human ALG14 293 Cell Lysate

• Cat.No.: ALG14-8907HCL

Specification

• Description: Antigen standard for asparagine-linked glycosylation 14 homolog (S. cerevisiae) (ALG14) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ALG14 asparagine-linked glycosylation 14 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: ALG14
• Synonyms: asparagine-linked glycosylation 14 homolog (S. cerevisiae); MGC19780; asparagine-linked glycosylation 14 homolog (yeast); UDP-N-acetylglucosamine transferase subunit ALG14 homolog; ALG14
• Gene ID: 199857
• mRNA Refseq: NM_144988.3
• Protein Refseq: NP_659425.1
• MIM: 612866
• UniProt ID: Q96F25
• Chromosome Location: 1p21.3
• Pathway: Asparagine N-linked glycosylation, organism-specific biosystem; Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; N-Glycan biosynthesis, organism-specific biosystem; N-Glycan biosynthesis, conserved biosystem; N-glycan precursor biosynthesis, organism-specific biosystem; N-glycan precursor biosynthesis, conserved biosystem

Reviews

10/26/2020

Catalyze the hydrolysis of clindamycin phosphate to clindamycin, and make it play an antibiotic role.

09/20/2019

ALG14 had high purity and no obvious impurities.

04/07/2019

Can be detected by AGAR electrophoresis method, it is convenient.

Q&As

What effect does overexpression or knockout of ALG14 have on cells?

Overexpression or knockout of ALG14 may negatively affect cellular glycosylation and the stability of ER and Golgi bodies, but the specific effects need to be further explored.

In what neurological diseases does ALG14 play a role?

The relationship between ALG14 and neurological diseases needs further study, but it is known to be associated with diseases such as intellectual deficiency and idiopathic epilepsy.

How neurological diseases can ALG14 mutations cause?

ALG14 mutations have been shown to be associated with a variety of neurological disorders such as intellectual impairment and idiopathic epilepsy.

In which metabolic pathways does ALG14 play a role?

ALG14 is involved in metabolic pathways such as glycosylation and glucose metabolism, and has important biological functions.

What is the position of ALG14 in cellular processes?

ALG14 is mainly distributed in the endoplasmic reticulum (ER) and the Golgi apparatus.

How biological processes does ALG14 participate in?

ALG14 is involved in several biological processes including protein glycosylation, ER and Golgi body sugar metabolism.

How does ALG14 regulate glycosylation?

ALG14, as a member of UDP-glucose-glycosyltransferase, is involved in the synthesis and modification of sugar chains during glycosylation, and plays an important role in the glycosylation and modification of post-translational mitochondrial proteins and plasma membrane proteins.

What is the sugar chain selectivity of ALG14?

This protein can selectively catalyze the specific chemical structure of sugar chains, thereby affecting the biological properties and function of proteins.

What are the molecular biological characteristics of ALG14?

This is an important UDP-glucose-glycosyltransferase. It plays a very important role in protein glycosylation and cell growth.

To which family members is ALG14 functionally similar?

As a member of the UDP-glucose-glycosyltransferase family, ALG14 is functionally similar to ALG13, ALG1 and other family members.

Which non-cellular autophagy pathways are involved in ALG14?

Although the function of ALG14 in the non-cellular autophagy pathway has not been clearly studied, there are indications that it may be involved in the TCA cycle and gluconeogenic pathway.

What effect does ALG14 have on the structure, length and branching of sugar chains?

This protein can influence the specific structure, length and branching of sugar chains through selective glycosylation reactions.

What is the interacting protein of ALG14?

ALG14 interacts with glycosylation enzymes such as ALG13, ALG1 and mannosyltransferase to jointly regulate the glycosylation process of proteins.

Does ALG14 participate in mitochondrial glucose metabolism?

ALG14 may be involved in mitochondrial glucose metabolism, although more research is needed to investigate the specific mechanism.

What is the relationship between ALG14 and the occurrence and development of diabetes?

There is currently no direct evidence that ALG14 is associated with the onset and development of diabetes, but glycosylation plays a key role in metabolism and disease development, so it may be involved in diabetes.

Is ALG14 involved in the occurrence of neurodegenerative diseases?

No studies have shown that ALG14 is directly related to the occurrence of neurodegenerative diseases, but glycosylation plays a crucial role in the development and function of the nervous system, so it may be related to neurodegenerative diseases.

Which cell signaling molecules are involved in ALG14?

The regulates the expression of glycosylase and the balance of glycosylation reaction by interacting with other glycosylated enzymes such as ALG13 and ALG1.

What are the effects of ALG14 inhibitors?

Several inhibitors have been developed to target ALG14, including inhibiting its glycosylation activity and regulating its interactions with other glycosylated enzymes.