Recombinant Human LH protein, His-GST-tagged

• Cat.No.: LH-533H
• Product Overview: Recombinant Human LH protein(Ala25~Ser116+GGGGS*3+Ser21~Leu141), fused to N-terminal His tag and GST tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His-GST
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
• Molecular Mass: 54kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ala25~Ser116+GGGGS*3+Ser21~Leu141
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 80%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Storage: Avoid repeated freeze/thaw cycles.
  Store at 2-8ºC for one month.
  Aliquot and store at -80ºC for 12 months.
• Reconstitution: Reconstitute in ddH2O to a concentration of 0-0.3 mg/mL. Do not vortex.

Reviews

07/11/2021

Excellent performance in the EMSA experiment.

08/14/2020

Useful in experimental models of calcium deficiency in mice.

09/15/2019

Apply for yeast double hybrid experiment.

04/19/2018

Effectively promote cell proliferation and division.

12/11/2016

None cytotoxicity and is beneficial to promote cell growth.

Q&As

Can patients with osteoporosis receive LHRH antagonist therapy?

There can be loss of bone mineral density so men at risk of osteoporosis should be carefully monitored or treated with bisphosphonates.

Is the antagonistic reaction of LH inhibitors reversible?

Inhibitors ablation are reversible.

Can LH drive proliferation, differentiation, and survival of multiple cell types?

LH activates extracellular signal-regulated kinase (ERK) and protein kinase B (PKB/AKT) to drive cell proliferation, differentiation and survival in a variety of cell types.

Can the LH signaling pathway be blocked with protein kinase A (PKA) inhibitors?

Yes,you can.Activated LHCGR couples to Gαs G-proteins to initiate the adenylyl cyclase, cAMP and protein kinase A (PKA) signaling pathway.

By which receptor does LH act?

LH act through the luteinizing hormone choriogonadotropin receptor (LHCGR).

Why does hormone therapy produce resistance?

Mechanisms include increased sensitivity of hormone receptors, hormone receptor variants, pathway activation without exposure to hormones (constitutive activation) or upregulated downstream survival pathways.

Can postmenopausal women achieve estrogen suppression without ovarian radiation or surgical removal?

Using a GnRH (or LH-releasing hormone (LHRH)) agonist (GnRHa,LHRHa) such as goserelin or leuprorelin, usually as a monthly depot injection.

What are the advantages of hormone therapy for cancer?

Hormone therapy can be used to reduce the size of the primary cancer before radical surgery or radiotherapy, or to reduce the risk of recurrence.

Can I get calcium by increasing my LH level?

At high hormone and receptor levels, LHCGR couples to Gαq G-proteins to activate phospholipase C and inositol phosphate signaling to increase intracellular Ca2+.

Does LH have the same structure as other gonadotropins?

Like all other gonadotropin hormones, are heterodimers consisting of two monomeric glycoproteins that combine to make a functional protein.They share a common α-subunit, with structural and functional specificity conferred through differing β-subunits.