Recombinant Human Sulfatase Modifying Factor 1, His-tagged

• Cat.No.: SUMF1-1539H
• Product Overview: Recombinant human SUMF1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Cat. No.: SUMF1-1539H
• Description: SUMF1 (Sulfatase-modifying factor 1) belongs to the SUMF family. SUMF1 is an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also known as C-alpha-formylglycine. Mutations in this gene cause multiple sulfatase deficiency, a lysosomal storage disorder.
• Form: Liquid. In 20mM Tris-HCl, pH8.0, 2M Urea, 20%Glycerol, 2mM DTT
• Molecular Weight: 34.1kDa (304aa)
• Purity: > 85% by SDS - PAGE
• Concentration: 0.5mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MVPIPAGVFT MGTDDPQIKQ DGEAPARRVT IDAFYMDAYE VSNTEFEKFV NSTGYLTEAE KFGDSFVFEG MLSEQVKTNI QQAVAAAPWW LPVKGANWRH PEGPDSTILH RPDHPVLHVS WNDAVAYCTW AGKRLPTEAE WEYSCRGGLH NRLFPWGNKL QPKGQHYANI WQGEFPVTNT GEDGFQGTAP VDAFPPNGYG LYNIVGNAWE WTSDWWTVHH SVEETLNPKG PPSGKDRVKK GGSYMCHRSY CYRYRCAARS QNTPDSSASN LGFRCAADRL PTMD
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Lysosome

Gene Information

• Gene Name: SUMF1 sulfatase modifying factor 1 [ Homo sapiens ]
• Official Symbol: SUMF1
• Synonyms: SUMF1; FGE; UNQ3037; AAPA3037; MGC131853; MGC150436; sulfatase-modifying factor 1; OTTHUMP00000115300; OTTHUMP00000207086; OTTHUMP00000207089; FGly-generating enzyme; C-alpha-formylglycine-generating enzyme 1; sulfatase modifying factor 1; OTTHUMP00000207088; EC 1.8.99
• Gene ID: 285362
• mRNA Refseq: NM_182760
• Protein Refseq: NP_877437
• MIM: 607939
• UniProt ID: Q8NBK3
• Chromosome Location: 3p26.1
• Function: binding; metal ion binding; oxidoreductase activity; protein homodimerization activity

Reviews

02/10/2022

Expert protein engineering support, an invaluable resource for our project.

06/14/2019

Consistent western blot results, a lab necessity for our research.

05/17/2019

Great protein crystallization service, vital for our structural biology work.

Q&As

What is the impact of SUMF1 on lysosomal function and storage diseases?

SUMF1 plays a role in lysosomal function, with implications for lysosomal storage diseases.

How does SUMF1 influence bone development and skeletal disorders?

SUMF1 influences bone growth and development, potentially affecting skeletal health and disorders.

What role does SUMF1 play in the degradation of glycosaminoglycans and mucopolysaccharides?

SUMF1 aids in the degradation of glycosaminoglycans, crucial for the breakdown of these complex molecules.

What are the mechanisms by which SUMF1 modulates cellular detoxification processes?

SUMF1 contributes to cellular detoxification, aiding in the breakdown of potentially harmful substances.

How do alterations in SUMF1 expression or function affect neurological development and function?

Variations in SUMF1 activity can impact neurological development and function, influencing brain health and disease.

How does SUMF1 deficiency contribute to the development of multiple sulfatase deficiency (MSD) and related symptoms?

SUMF1 deficiency leads to multiple sulfatase deficiency, disrupting normal metabolism and causing various symptoms.

How does SUMF1 (Sulfatase Modifying Factor 1) activate sulfatase enzymes involved in cellular metabolism?

SUMF1 is essential for the activation of sulfatase enzymes, impacting their role in cellular metabolism.