Recombinant Mouse ALG5 Protein, His (Fc)-Avi-tagged

• Cat.No.: ALG5-468M
• Product Overview: Recombinant Mouse ALG5 with His (Fc)-Avi tag was expressed and purified

Specification

• Source: HEK293
• Species: Mouse
• Tag: His (Fc)-Avi
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: ≥85% by SDS-PAGE
• Stability: Stable for at least 6 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: Alg5 asparagine-linked glycosylation 5 (dolichyl-phosphate beta-glucosyltransferase) [ Mus musculus ]
• Official Symbol: ALG5
• Gene ID: 66248
• mRNA Refseq: NM_025442.3
• Protein Refseq: NP_079718.1
• UniProt ID: Q9DB25

Reviews

01/30/2023

ALG5 has been found to induce specific immune responses in immunotherapy, which has potential value for disease treatment.

09/27/2022

ALG5 was found to efficiently load and release drugs in drug delivery systems.

07/23/2022

In vaccine studies, ALG5 has been found to induce immunoprotective effects and has potential for disease prevention.

08/27/2020

In genetic engineering studies, ALG5 has been found to have good application potential in protein engineering and recombinant expression.

Q&As

Which metabolic pathways are involved in ALG5?

It is involved in metabolic pathways such as glycosylation and glucose metabolism.

How is ALG5 distributed in mammalian cells?

It is mainly distributed in the endoplasmic reticulum and Golgi apparatus in mammalian cells.

What effect does overexpression or knockout of ALG5 have on cells?

Overexpression or knockout of ALG5 can negatively affect the glycosylation of cells and the stability of ER and Golgi bodies, but the specific effects need to be further explored.

What are the molecular biological characteristics of ALG5?

ALG5 plays an important role in protein glycosylation, participating in the synthesis and modification of sugar chains.

What are the interacting proteins of ALG5?

ALG5 interacts with members of the glycosylase family such as ALG3, ALG2 and other related proteins to jointly regulate the glycosylation process of proteins.

What non-cellular autophagy pathways are involved in ALG5?

Although there are currently no studies demonstrating the function of ALG5 in non-cellular autophagy pathways, there are indications that it may be involved in the TCA cycle and gluconeogenic pathways.

What biological processes does ALG5 participate in?

ALG5 is involved in several biological processes including protein glycosylation, ER and Golgi body sugar metabolism.

What is the gene structure and regulation of ALG5?

The ALG5 gene contains 9 exons and its expression is influenced by multiple regulatory elements and signaling pathways.

What are the effects of ALG5 inhibitors?

A number of inhibitors have been developed that target ALG5 and inhibit its glycosylation activity, thereby interfering with its function in cellular processes.

In what metabolic diseases does ALG5 play a role?

ALG5 mutation may lead to the occurrence and development of some metabolic diseases such as CDG, and further investigation is needed to confirm its role in metabolic diseases.

In which cancers does ALG5 play a role?

At present, there is no direct evidence that ALG5 is related to the occurrence and development of cancer, but glycosylation plays an important role in metabolism and disease development, so it may be relevant to related diseases.

Does ALG5 participate in mitochondrial glucose metabolism?

No studies have confirmed whether ALG5 is involved in mitochondrial glucose metabolism, and more studies are needed to prove it.

Does ALG5 mutation have an adverse effect on embryonic development?

The mutations can have adverse effects on embryonic development and may therefore be associated with a number of fetal diseases.

Which cell signaling molecules are involved in ALG5?

The regulates the expression of glycosylase and the balance of glycosylation reaction by interacting with other glycosylated enzymes such as ALG3 and ALG2.

Which diseases do ALG5 mutations cause?

This protein mutation may be associated with some genetic diseases, such as CDG (Congenital Disorder of Glycosylation).

In what neurological diseases does ALG5 play a role?

ALG5 plays an important role in some neurological diseases, such as central nervous system dysplasia, leading to symptoms such as mental retardation.

What is the sugar chain selectivity of ALG5?

ALG5 selectively catalyzes the synthesis and modification of sugar chains and plays an important role in the binding selectivity of glycosylase family members to substrate proteins.