Active Recombinant Human ADAMTS5, His-tagged

• Cat.No.: ADAMTS5-534H
• Product Overview: Recombinant Human ADAMTS5 was expressed in abaculovirussystem and purified from insect cells. The enzyme contains the catalytic domain, the disintegrin domain and the thrombospondin type-1 motif of full-length ADAMTS-5 followed by a C-terminal His tag, 50 kDa.

Specification

• Cat. No.: ADAMTS5-534H
• Description: Aggrecanases belong to a family of ADAMTS proteases (ADAMTS = A disintegrin and metalloprotease with thrombospondin motifs). ADAMTS enzymes are comprised of an N-terminal prodomain, a catalytic domain, a disintegrin domain, one or more thrombospondin (TS) motifs, a cysteine-rich domain and a spacer domain of variable length. The prodomains are cleaved off intracellularly and the enzymes are processed further from their C-terminal ends after secretion. Aggrecanases are responsible for aggrecan degradation in osteoarthritic joints. ADAMTS-5 was found to be the principal aggrecanase in mouse cartilage.
• Source: Baculovirus.
• Activity: Activity (mU/mg) is specified on the vial.
• Purity: ≥ 50%.
• Unit Definition: One milliunit of protease hydrolyzes 1 nanomole of aggrecan interglobular domain substrate per minute at pH 7.5 at 37°C.
• Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2, 50 mM Imidazol, 0.05% Brij-35.
• Application: Used as a positive control in FRET-based and aggrecan enzyme activity assays, Western blot, IP, and ELISA.
• Storage: Store at -80°C. Avoid multiple thaw-freeze cycles.

Gene Information

• Gene Name: ADAMTS5 ADAM metallopeptidase with thrombospondin type 1 motif, 5 [ Homo sapiens ]
• Synonyms: ADAM metallopeptidase with thrombospondin type 1 motif, 5; ADMP-2; ADAMTS11; FLJ36738; ADAMTS5; a disintegrin and metalloproteinase with thrombospondin motifs 5; ADAM-TS5; ADAM-TS 5; ADAMTS-11;ADAM-TS 11; aggrecanase-2; a disintegrin and metalloproteinase with thrombospondin motifs 11; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2); EC 3.4.24.-
• Gene ID: 11096
• mRNA Refseq: NM_007038
• Protein Refseq: NP_008969
• MIM: 605007
• Chromosome Location: 21q21.3
• Function: integrin binding; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding

Reviews

06/24/2020

The staff was very nice, communicated with me positively and solved a lot of technical problems.

06/19/2020

The ADAMTS5 proteins purchased from your company is suitable for ELISA.

06/17/2020

Fast and timely shipment helped me to get satisfactory experimental results in short time.

Q&As

Do you offer ADAMTS5 fused with other tags?

We can provide you with the most suitable labels according to your experimental needs.

ADAMTS5 is used in studies of which diseases?

ADAMTS5 is used in the study of oral squamous cell carcinoma, gastric cancer, and many other cancers.

How does ADAMTS5 relate to Aggrecan?

ADAMTS5 can cut Aggrecan.

What is the structure domain of ADAMTS5?

DAMTS5 is composed of a signal peptide, a pro-domain, a catalytic metalloproteinase domain, a disintegrin-like domain, and other C-terminal ancillary domains (a central thrombospondin type 1 sequence repeat (central TSR) motif, a cysteine-rich domain, a spacer region, and an additional TSR motif).

What biological processes is ADAMTS5 involved in?

ADAMTS5 is involved in the degradation of polyproteoglycans.