Recombinant Human ALDH1A3 293 Cell Lysate

• Cat.No.: ALDH1A3-8919HCL

Specification

• Description: Antigen standard for aldehyde dehydrogenase 1 family, member A3 (ALDH1A3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ALDH1A3 aldehyde dehydrogenase 1 family, member A3 [ Homo sapiens ]
• Official Symbol: ALDH1A3
• Synonyms: ALDH1A3; aldehyde dehydrogenase 1 family, member A3; ALDH6; aldehyde dehydrogenase family 1 member A3; RALDH3; retinaldehyde dehydrogenase 3; RALDH-3; aldehyde dehydrogenase 6; acetaldehyde dehydrogenase 6; ALDH1A6
• Gene ID: 220
• mRNA Refseq: NM_000693
• Protein Refseq: NP_000684
• MIM: 600463
• UniProt ID: P47895
• Chromosome Location: 15q26
• Pathway: Drug metabolism - cytochrome P450, organism-specific biosystem; Drug metabolism - cytochrome P450, conserved biosystem; Glycolysis / Gluconeogenesis, organism-specific biosystem; Glycolysis / Gluconeogenesis, conserved biosystem; Histidine metabolism, organism-specific biosystem; Histidine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: NAD+ binding; aldehyde dehydrogenase (NAD) activity; aldehyde dehydrogenase [NAD(P)+] activity; oxidoreductase activity; protein homodimerization activity; thyroid hormone binding

Reviews

04/27/2022

The combination of high-quality ALDH1A3 protein and excellent technical support provided by the manufacturer makes them the ideal choice for my research.

11/05/2020

The manufacturer's extensive experience and expertise in the field of ALDH1A3 protein research make them a trusted partner.

10/14/2016

The manufacturer's commitment to providing excellent technical support is highly valuable.

Q&As

How is ALDH1A3 protein clinically relevant?

ALDH1A3 has been implicated in cancer stem cell maintenance and differentiation, making it a potential target for cancer therapy.

Can ALDH1A3 be a potential therapeutic target beyond cancer?

ALDH1A3 has implications beyond cancer, and its role in retinoic acid metabolism suggests potential therapeutic applications in developmental disorders and other diseases.

How does ALDH1A3 contribute to the epithelial-mesenchymal transition (EMT) process?

ALDH1A3 is involved in the EMT process, a crucial step in cancer metastasis, highlighting its significance in tumor invasion and spread.

In which cancers is ALDH1A3 protein overexpressed?

ALDH1A3 overexpression has been observed in various cancers, including breast, lung, ovarian, and pancreatic cancers.

Are there any ongoing clinical trials targeting ALDH1A3?

Clinical trials are exploring the therapeutic potential of targeting ALDH1A3, evaluating the safety and efficacy of novel drugs in cancer patients.