Recombinant Human ALDH8A1 293 Cell Lysate

• Cat.No.: ALDH8A1-8914HCL

Specification

• Description: Antigen standard for aldehyde dehydrogenase 8 family, member A1 (ALDH8A1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ALDH8A1 aldehyde dehydrogenase 8 family, member A1 [ Homo sapiens ]
• Official Symbol: ALDH8A1
• Synonyms: ALDH8A1; aldehyde dehydrogenase 8 family, member A1; aldehyde dehydrogenase family 8 member A1; ALDH12; aldehyde dehydrogenase 12; aldehyde dehydrogenase family protein; DJ352A20.2; MGC138650; DKFZp779D2315
• Gene ID: 64577
• mRNA Refseq: NM_001193480
• Protein Refseq: NP_001180409
• MIM: 606467
• UniProt ID: Q9H2A2
• Chromosome Location: 6q24.1-q25.1
• Function: oxidoreductase activity; retinal dehydrogenase activity

Reviews

10/26/2019

Researchers utilizing the Aldh8a1 Protein can rely on its robust and reproducible performance in a variety of experimental settings.

12/20/2018

This protein has proven to be of great utility in protein electron microscopy structure analysis.

04/09/2017

broad applicability make it a valuable asset for a wide range of research endeavors.

04/27/2016

The Aldh8a1 Protein comes highly recommended for its exceptional performance, particularly in ELISA assays.

Q&As

Are there any therapies or treatments specifically targeting the ALDH8A1 protein?

As of now, there are no specific therapies or treatments targeting the ALDH8A1 protein. The focus is primarily on managing and treating the developmental disorders or diseases associated with ALDH8A1 gene mutations, such as Antley-Bixler syndrome, through supportive and symptomatic care.

What are the potential roles of the ALDH8A1 protein in diseases other than Antley-Bixler syndrome?

While Antley-Bixler syndrome is the most well-known disease associated with ALDH8A1 gene mutations, there is increasing evidence suggesting potential roles for ALDH8A1 in other diseases. For example, it has been implicated in some forms of cancer, including ovarian and gastric cancer. However, further research is necessary to fully understand its involvement in these conditions.

Are there any other known functions or roles of the ALDH8A1 protein?

While the primary function of the ALDH8A1 protein is the conversion of retinaldehyde to retinoic acid, the full extent of its functions is still being investigated. Some studies suggest its involvement in the detoxification of certain aldehydes, but further research is needed to fully understand its roles.

Where is the ALDH8A1 protein expressed in the body?

The ALDH8A1 protein is expressed in various tissues and organs of the body. It is found in high levels in the liver, as well as in other tissues such as the brain, kidney, lung, and small intestine.

How is the ALDH8A1 protein regulated?

The regulation of ALDH8A1 expression and activity is not fully understood. However, it is known that its expression can be influenced by various factors, including hormones, vitamin A levels, and developmental signaling pathways. Further research is needed to elucidate the precise mechanisms of its regulation.

Are there any known interacting partners or proteins that interact with ALDH8A1?

The specific protein-protein interactions involving ALDH8A1 are not well-characterized. However, as an enzyme involved in retinoic acid metabolism, it likely interacts with other proteins in the retinoid signaling pathway. Some studies suggest that ALDH8A1 may interact with cellular retinoic acid-binding proteins (CRABP) and retinoic acid receptor (RAR) proteins, but more research is needed to confirm and fully understand these interactions.

Are there any inhibitors or activators of the ALDH8A1 protein?

Some compounds have been identified as possible inhibitors or activators of ALDH8A1 activity. For example, it has been found that disulfiram, a drug used to treat alcoholism, can inhibit ALDH8A1 activity. However, the therapeutic potential of these compounds in relation to ALDH8A1 function is not yet fully understood.

How is the ALDH8A1 protein related to human health?

Mutations or dysregulation in the ALDH8A1 gene and protein have been associated with certain developmental disorders and diseases. One example is Antley-Bixler syndrome, a rare genetic disorder characterized by craniosynostosis (premature fusion of the skull bones) and other skeletal and genital abnormalities. ALDH8A1 mutations have been found in individuals with this condition.