Recombinant Human SLC5A2 293 Cell Lysate

• Cat.No.: SLC5A2-1709HCL

Specification

• Description: Antigen standard for solute carrier family 5 (sodium/glucose cotransporter), member 2 (SLC5A2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SLC5A2 solute carrier family 5 (sodium/glucose cotransporter), member 2 [ Homo sapiens ]
• Official Symbol: SLC5A2
• Synonyms: SLC5A2; solute carrier family 5 (sodium/glucose cotransporter), member 2; SGLT2; sodium/glucose cotransporter 2; Na(+)/glucose cotransporter 2; solute carrier family 5 member 2; low affinity sodium-glucose cotransporter; solute carrier family 5 (sodium/glucose transporter), member 2
• Gene ID: 6524
• mRNA Refseq: NM_003041
• Protein Refseq: NP_003032
• MIM: 182381
• UniProt ID: P31639
• Chromosome Location: 16p12-p11
• Pathway: Na+-dependent glucose transporters, organism-specific biosystem; SLC-mediated transmembrane transport, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem; Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds, organism-specific biosystem
• Function: low-affinity glucose:sodium symporter activity; symporter activity; transporter activity

Reviews

05/04/2022

Its stability and compatibility make it an excellent tool for visualizing and characterizing protein structures at high resolution.

03/18/2022

The SLC5A2 protein stands out for its exceptional quality, making it an excellent choice to meet my experimental requirements.

05/01/2016

the SLC5A2 protein's compatibility with various experimental techniques adds to its versatility.

Q&As

Is there any potential for SLC5A2-targeted therapy in other organ systems?

SLC5A2 inhibitors may have implications beyond diabetes, including potential cardiovascular and renal protective effects.

How does the function of the SLC5A2 protein affect glucose homeostasis?

The SLC5A2 protein plays a crucial role in maintaining normal blood glucose levels by reabsorbing filtered glucose from the urine back into the bloodstream.

Are there any diagnostic tests for assessing SLC5A2-related conditions?

Genetic testing for SLC5A2 mutations can help identify individuals with renal glycosuria or other related conditions.

How do pharmaceutical companies target the SLC5A2 protein for drug development?

Pharmaceutical companies are developing SGLT2 inhibitors, which can selectively block the activity of the SLC5A2 protein to reduce glucose reabsorption in the kidneys.

Can SLC5A2 mutations lead to any adverse effects?

In individuals with SLC5A2 mutations, the increased excretion of glucose in the urine may predispose them to urinary tract infections and dehydration.