Recombinant Human TDP1, His-tagged

• Cat.No.: TDP1-7250H
• Product Overview: Recombinant human TDP1, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Description: Tyrosyl-DNA phosphodiesterase 1, also known as TDP1, is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol.
• Molecular Mass: 35.8 kDa
• Protein length: 318aa
• Purity: > 90% by SDS - PAGE.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay).

Gene Information

• Gene Name: TDP1 tyrosyl-DNA phosphodiesterase 1 [ Homo sapiens ]
• Official Symbol: TDP1
• Synonyms: TDP1; tyrosyl-DNA phosphodiesterase 1; FLJ11090; SCAN1; tyr-DNA phosphodiesterase 1; MGC104252
• Gene ID: 55775
• mRNA Refseq: NM_001008744
• Protein Refseq: NP_001008744
• MIM: 607198
• UniProt ID: Q9NUW8
• Chromosome Location: 14q32.11
• Pathway: DNA Repair, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Nonhomologous End-joining (NHEJ), organism-specific biosystem; Processing of DNA ends prior to end rejoining, organism-specific biosystem
• Function: 3-tyrosyl-DNA phosphodiesterase activity; double-stranded DNA binding; exonuclease activity; hydrolase activity; phosphoric diester hydrolase activity; protein binding; single-stranded DNA binding

Reviews

07/14/2022

Very professional customer service, fully understanding the needs of the customer and giving professional advice.

06/21/2021

The technical expert will follow up on the progress of my experiments and provide constructive advice on the problems that arise in my experiments.

05/09/2021

Fast and timely shipment helped me to get satisfactory experimental results in short time.

Q&As

What are the effects of inhibiting TDP1 expression?

Inhibition of TDP1 expression induces apoptosis in gastric cancer cells.

What diseases has TDP1 been closely associated with the study of?

TDP1 has been closely associated with the study of diseases such as gastric cancer and lung cancer.

How does the expression level of TDP1 change in lung adenocarcinoma tissues?

Lung adenocarcinoma tissues expressed higher levels of TDP1 compared to paraneoplastic and normal tissues.

What is the biological function of TDP1?

TDP1 is involved in DNA damage repair and plays a driving role in tumorigenesis and development.