Recombinant Human TDP1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TDP1-2334H
• Product Overview: TDP1 MS Standard C13 and N15-labeled recombinant protein (NP_001008744) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1).
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 68.4 kDa
• AA Sequence: MSQEGDYGRWTISSSDESEEEKPKP DKPSTSSLLCARQGAANEPRYTCSE AQKAAHKRKISPVKFSNTDSVLPPK RQKSGSQEDLGWCLSSSDDELQPEM PQKQAEKVVIKKEKDISAPNDGTAQ RTENHGAPACHRLKEEEDEYETSGE GQDIWDMLDKGNPFQFYLTRVSGVK PKYNSGALHIKDILSPLFGTLVSSA QFNYCFDVDWLVKQYPPEFRKKPIL LVHGDKREAKAHLHAQAKPYENISL CQAKLDIAFGTHHTKMMLLLYEEGL RVVIHTSNLIHADWHQKTQGIWLSP LYPRIADGTHKSGESPTHFKADLIS YLMAYNAPSLKEWIDVIHKHDLSET NVYLIGSTPGRFQGSQKDNWGHFRL KKLLKDHASSMPNAESWPVVGQFSS VGSLGADESKWLCSEFKESMLTLGK ESKTPGKSSVPLYLIYPSVENVRTS LEGYPAGGSLPYSIQTAEKQNWLHS YFHKWSAETSGRSNAMPHIKTYMRP SPDFSKIAWFLVTSANLSKAAWGAL EKNGTQLMIRSYELGVLFLPSAFGL DSFKVKQKFFAGSQEPMATFPVPYD LPPELYGSKDRPWIWNIPYVKAPDT HGNMWVPSTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TDP1 tyrosyl-DNA phosphodiesterase 1 [ Homo sapiens (human) ]
• Official Symbol: TDP1
• Synonyms: TDP1; tyrosyl-DNA phosphodiesterase 1; FLJ11090; SCAN1; tyr-DNA phosphodiesterase 1; MGC104252
• Gene ID: 55775
• mRNA Refseq: NM_001008744
• Protein Refseq: NP_001008744
• MIM: 607198
• UniProt ID: Q9NUW8

Reviews

07/14/2022

Very professional customer service, fully understanding the needs of the customer and giving professional advice.

06/21/2021

The technical expert will follow up on the progress of my experiments and provide constructive advice on the problems that arise in my experiments.

05/09/2021

Fast and timely shipment helped me to get satisfactory experimental results in short time.

Q&As

What are the effects of inhibiting TDP1 expression?

Inhibition of TDP1 expression induces apoptosis in gastric cancer cells.

What diseases has TDP1 been closely associated with the study of?

TDP1 has been closely associated with the study of diseases such as gastric cancer and lung cancer.

How does the expression level of TDP1 change in lung adenocarcinoma tissues?

Lung adenocarcinoma tissues expressed higher levels of TDP1 compared to paraneoplastic and normal tissues.

What is the biological function of TDP1?

TDP1 is involved in DNA damage repair and plays a driving role in tumorigenesis and development.