Brief Introduction
Creative BioMart provides you with antibodies targeting various histone post-translational modifications (PTMs), as well as antibodies targeting modifying enzymes or reader domain-containing proteins involved in the dynamic changes of various types of histone PTMs.
Histone PTM plays a significant role in epigenetic regulation. Histones undergo PTM, affecting their interaction with DNA. These histone modifications make up the histone code, which determines the transcriptional status of local genomic regions. Detection of histone modifications can reveal the gene activation status, as well as the location of promoters, enhancers and other gene regulatory elements.
| Classic Histone PTM in Epigenetics | |
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One or more methyl groups are added to lysine (K) or arginine (R) residues in histone tails. Histone methyltransferase (HMT) is specific for the histone residues and mono-, di-, or trimethylation. |
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Acetyl groups from acetyl-CoA are added to specific histone lysine (K) residues by histone acetyltransferases (HATs) and deacetylation is catalyzed by specific histone deacetylases (HDACs). In general, histone acetylation promotes transcription, while deacetylation inhibits transcription. |
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Kinases catalyze the binding of phosphate groups to specific serine (S), threonine (T), or tyrosine (Y) residues at the tail of the histone, which is involved in the transcriptional activation of the gene. And dephosphorylation is catalyzed by phosphatases. |
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Ubiquitination is also an epigenetic mark. Protein ubiquitination is the process by which the carboxyl-terminus of the ubiquitin molecule binds to the lysine (K) residue of the protein. Histone ubiquitination requires a series of enzymes E1, E2, and E3, and ubiquitin is removed by deubiquitinating enzymes (DUBs). |
Histone PTM is an extremely challenging target for antibody recognition. Our monoclonal histone PTM antibodies have extraordinary specificity for specific PTMs and are powerful tools for the detection and characterization of epigenetic targets. These antibodies have been validated by a variety of tests, such as peptide array screening, western blot, chromatin immunoprecipitation (ChIP), and dot blot detection, to select antibodies that do not cross-react with other histone modifications.
In addition to antibodies against various histone PTMs, we also produce a large number of antibodies that target proteins/enzymes involved in the dynamic process of histone modification. These antibody products can be used in many antibody-based experimental techniques to provide reliable results for your epigenetic research. If you do not find a suitable antibody in our product list, we can also produce or modify the antibody under your requirements.
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