DNA Modification & Methylation


Brief Introduction


DNA Modification & Methylation

DNA methylation (5-methylcytosine, 5mC) is a significant epigenetic modification that regulates transcription and thus affects gene expression. DNA cytosine methylation is established by adding a methyl group to the fifth carbon atom of cytosine by DNA methyltransferase (DNMT). DNA methylation is in a dynamic process, in which DNA methylation can be established and maintained by DNMTs and demethylated by DNA demethylases. In addition to 5mC, DNA methylation variants have recently been found in mammals, including 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). Particularly, 5hmC has been proved to be not only an intermediate of DNA demethylation, but also a crucial epigenetic marker enriched in genomes.

Creative BioMart not only provides you with analysis services of DNA methylation and DNA hydroxymethylation based on various technologies, but also provides a variety of research kits involved in the study of 5mC and 5hmC to help you understand DNA methylation/hydroxymethylation information at different levels.

  1. DNA Bisulfite Conversion
Bisulfite conversion is a widely used method for converting a sample with DNA methylation markers into a suitable downstream analysis template. Its principle is based on the chemical conversion of unmethylated cytosine to uracil, while methylated cytosine is not affected by this conversion, thus various analyses of the changed sequence can be performed to retrieve this information. The combination of bisulfite conversion with other downstream technologies allows methylation analysis at a single nucleotide resolution. Research kits provided by Creative BioMart can help you perform efficient and accurate bisulfite conversion of various sample types. The converted DNA can be used for multiple downstream applications, such as PCR, microarray, and next generation sequencing, etc.
  1. DNA Methylation Quantification
DNA methylation plays an important role in the occurrence and development of many cancers. Therefore, quantitative analysis of 5-mC content or methylation in tumor cells can provide useful information for the detection and analysis of the disease. Creative BioMart’s kits are based on various detection methods, allowing DNA methylation status to be detected from multiple species (mammalian cells, plants, fungi, bacteria and viruses) and from multiple sample types (genomic DNA, cultured cells, fresh and frozen tissues, paraffin-embedded tissues, plasma/serum samples, and body fluid samples, etc.). Moreover, we have also launched several kits for measuring 5hmC content.
  1. Methylated DNA Immunoprecipitation (MeDIP)
Creative BioMart offers you multiple research kits of MeDIP, which is an antibody-based technology for enriching and capturing methylated DNA fragments for gene-specific DNA methylation studies on a genome wide scale. The DNA obtained after MeDIP enrichment can be used in multiple downstream applications, such as MeDIP-Seq, MeDIP-qPCR, and MeDIP-chip. In addition, our hMeDIP kit are suitable for DNA hydroxymethylation analysis.
  1. DNA Methylation Standard
A methylation-positive control is an indispensable tool for successful gene methylation research. It can be used with other kits that detect DNA modification or DNA methylation.
  1. DNA Methyltransferase & Demethylase Assay Kits
DNMTs are key enzymes for establishing and maintaining DNA methylation. And it is further found that DNA hydroxymethylation caused by 5mC oxidation by TET family enzymes is related to the control of gene expression and is an important DNA demethylation process. Assays measuring the activity/inhibition of DNMT and other enzymes involved in the DNA methylation cycle facilitate the discovery of related drugs.

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