Cryo-EM for Chromatin Remodeling Complex


Chromatin remodeling factor is an important component of epigenetic regulation. In eukaryotic cells, ATP-dependent chromatin remodeling factors regulate chromatin structure by altering the assembly, disassembly, and rearrangement of nucleosomes on chromatin, thereby improving the local accessibility of transcription-related factors such as transcription factors to DNA.

Therefore, the three-dimensional (3D) structure analysis of various chromatin remodeling complexes (CRC) will help us better understand their specific biological functions and mechanisms. However, the CRC composed of multi-subunits is usually of high molecular mass and inevitably has structural changes when it plays a chromatin remodeling role in the cell, so it is relatively difficult to analyze the 3D structure of CRC. Currently, the research on the 3D structure analysis of remodeling factors and complexes mainly based on the crystal analysis of each subunit in the complex and the electron microscopy analysis of the whole or a part of the complex, especially in recent years, the resolution of cryo-electron microscopy (cryo-EM) has reached a very high level.

As part of Creative BioMart, a protein research expert, we have extensive experience in using cryo-EM to study the structure of biomacromolecules and their complexes. Creative BioMart currently has the ability to provide cryo-EM service to support the study of CRC.

Examples of CRC structures determined by cryo-EM.

Figure 1. Examples of CRC structures determined by cryo-EM. (Eustermann S.; et al. 2018)

Advantages of Our Service

  1. A preferred method for target proteins that are intractable to crystallization
  2. Only small amount of protein sample required (mg scale) for EM studies
  3. Sample preparation by cryo-fixation to capture their near-native conformational states
  4. State-of-the-art cryo-EM platform with powerful software for data processing
  5. Single-particle reconstruction to build 3D structural models of target proteins at high resolution

Workflow of Cryo-EM for CRC Service at Creative BioMart

Workflow of Cryo-EM for CRC Service at Creative BioMart

Figure 2. Workflow of Cryo-EM for CRC Service at Creative BioMart

Creative BioMart will provide comprehensive support for your CRC research from sample preparation, EM imaging and data processing, as well as model building and refinement. Please feel free to contact us for a detailed quote.

Reference
1. Eustermann S.; et al. Structural basis for ATP-dependent chromatin remodeling by the INO80 complex. Nature. 2018, 556(7701): 386-390.

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