MeRIP-Seq Service


Creative BioMart is able to offer MeRIP-Seq (m6A-specific methylated RNA) service to help customers exploit RNA methylation patterns from MeRIP-seq high-throughput data, reveal the potential functions of mRNA methylation in regulating gene expression, splicing, and translation, and effectively guide an amount of basic research and the intervention of various diseases.

What Is m6A Methylation and MeRIP-Seq?

N6-methyladenosine (m6A) is the most common form of RNA methylation, accounting for 80%. m6A is dynamically reversible and has important research significance, since it plays a key role in post-transcriptional regulation, including regulation of gene expression, splicing, RNA editing, RNA stability, control of mRNA lifetime and degradation, and mediation of circular RNA translation. Therefore, in-depth study of m6A RNA methylation can help solve biological problems such as cell differentiation, biological growth and development, and disease development, and help drug developers design small molecules that regulate gene expression, and kill or control diseased cells. Methylated RNA immunoprecipitation sequening (MeRIP-seq) technology can be used to comprehensively study the post-transcriptional methylation modification of RNA, which is a key technology for epigenetic research.

Dynamically reversible process of m6A RNA methylation

Fig 1. Dynamically reversible process of m6A RNA methylation

MeRIP-seq technology combines methylated DNA immunoprecipitation (MeDIP) technology, RNA immunoprecipitation (RIP) technology and RNA sequencing (RNA-seq) technology to accurately detect RNA methylation in the whole transcriptome range. The methylated RNA-specific antibodies are incubated with randomly interrupted RNA fragments by immunoprecipitation, and the methylated fragments are captured for sequencing. At the same time, a control sample needs to be sequenced in parallel, and is used to eliminate the background in the process of capturing the methylated fragment. The sequence fragments in the immunoprecipitation (IP) sample and the control sample are then compared or localized to the reference genome/transcriptome to detect the RNA methylation site. The control sample measures the expression quantity of the corresponding RNA, which is essentially RNA-seq data.

MeRIP-Seq Workflow

Diagram of MeRIP-Seq workflow

Fig 2. Diagram of MeRIP-Seq workflow

Categories of MeRIP-Seq

Categories of MeRIP-Seq
  1. m6A whole transcriptome sequencing
  2. m6A circular RNA sequencing
  3. m6A mRNA sequencing
  4. m6A LncRNA sequencing

Features of MeRIP-Seq

  1. High flexibility: Direct sequencing of transcriptome methylated fragments of any species without known genomic sequence information.
  2. Wide detection range: Covering the whole transcriptome methylation area.
  3. High accuracy: Accurate positioning within the range of 100-200 bases of actual binding site.
  4. Digitized signal: Methylation fragments are directly quantified and sequenced, and there is no cross reaction and background noise caused by traditional chip hybridization signals.

Advantages of Creative BioMart

  1. One-stop service: Customers only need to provide cells, tissues or RNA. Creative BioMart completes the whole service process from MeRIP enrichment, cDNA library preparation, on-machine sequencing to data analysis.
  2. High specificity: Through optimized experimental procedures and commercialized antibodies to enrich methylated RNA fragments and help customers complete the transcriptome-wide RNA methylation study with extremely high efficiency and specificity at a lower cost.
  3. Strict quality control: Full-scale monitoring of MeRIP experiment to ensure that customers receive quality data.
  4. Professional bioinformatics analysis: A professional bioinformatics analysis team to help customers fully explore experimental data.

With experienced experts and fast-responding teams, Creative BioMart can provide high-quality MeRIP-Seq services, including sample standardization, library construction, deep sequencing, raw data quality control, and customized bioinformatics analysis, to ensure the objectives are met and expedite epigenetic research. If you have additional requirements or questions, please feel free to contact us.

Reference
1. Wang, X.; et al. N6-methyladenosine-dependent regulation of messenger RNA stability. Nature, 2014, 505(7481):117-20.
2. Liu, J. and Jia, G. Methylation Modifications in Eukaryotic Messenger RNA. J GENET GENOMICS, 2014, 41(1): 21-33.
3. Cao, G.; et al. Recent advances in dynamic m6A RNA modification. Open biology, 2016, 6(4): 160003.

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