In organisms, the expression of messenger RNA (mRNA) is different in different tissue parts, different developmental stages, or different environments. If you want to detect gene expression, study gene structure, gene function, mRNA selective splicing or predict novel transcripts, at Creative BioMart, our cost-effective mRNA sequencing service will meet your research needs in this series.

What Is mRNA Sequencing?

mRNA sequencing (mRNA-Seq) is a targeted RNA-Seq protocol that enriches for all polyadenylated (poly-A) transcripts of the transcriptome. Only around 1~2% of the entire transcriptome is comprised of poly-A tailed RNA, which is the coding part of the genome. mRNA-Seq enables the identification of novel transcripts, mRNA selective splicing, and gene fusion events. This technique also provides another affordable approach for gene expression quantification and differential gene expression analysis between sample groups. By targeting mRNA, sequencing depth is improved as resources are dedicated to the sequencing of coding genes. This makes identifying rare variants and low expressed mRNA transcripts easier. With high throughput and high sensitivity, it is a powerful tool for in-depth study of transcripts. At present, mRNA-Seq has been widely utilized in gene screening of animals and plants, as well as disease research. In mRNA-Seq, single RNA strands are converted to complementary DNAs (cDNA) and then turned into double stranded DNA before being sequenced. So, while the initial starting input material is RNA, material loaded on the sequencing instrument is DNA.

Figure 1. Schematic illustration of RNA-Seq

Applications of mRNA Sequencing

1. Functional annotation of coding genes
2. Gene expression profiling and identification of differentially expressed genes
3. Epigenetic mechanism of gene expression (transcriptome plus H3K36me3 and H3K4me3 marks)

Our Advantages

1. Tolerance of multiple sample types: we currently accept a wide range of biological and clinical samples including total RNA without degradation or DNA contamination, cells and tissues. Sample sources include human, animals, plants as well as microorganisms.
2. One-stop service: customers only need to provide samples, and we complete a full mRNA-Seq experiment, including project consultation, experimental design, experimental execution and data analysis, as well as troubleshooting during the experiment. The final report you will receive includes a complete experimental protocol, raw data, analytical data, and a summary of the result analysis.
3. Reliable data quality: Our data quality guarantee, as measured by the percentage of bases with a sequencing quality score above Q30 (PE 150, ≥80%; SE 50, ≥90%), exceeds Illumina’s official guarantee (PE 150, ≥75%; SE 50, ≥80%).
4. Professional bioinformatics analysis: our experienced bioinformatics specialists work closely with customers to provide both standard data analyses (such as mapping to reference genome, differential expression analysis, GO analysis for genes with significantly different expression level and KEGG metabolic pathways for genes with significantly different expression level) and customized bioinformatics analyses.

Workflow of mRNA Sequencing at Creative BioMart

Creative BioMart stringently controls each step in the mRNA-Seq workflow to ensure access to unsurpassed data quality.

Figure 2. Workflow of mRNA sequencing service at Creative BioMart

Creative BioMart creates a leading transcriptome sequencing laboratory using standardized experimental processes, advanced sequencing platforms and professional bioinformatics analysis, serving customers from all over the world and winning professional praise with high efficiency and high-quality technical services. In addition to mRNA-Seq, we also offer whole transcriptome, small non-coding RNA, circular RNA (circRNA) and long non-coding RNA (lncRNA) sequencing services. Please don’t hesitate to contact us for more information.

Reference
1. Kukurba K R, Montgomery S B. RNA sequencing and analysis. Cold Spring Harbor Protocols. 2015, 2015(11): 951.