Chromatin Analysis Service


It is well known that chromatin is a linear composite dynamic structure. The basic structural unit of chromatin is the nucleosome, which is composed of DNA and histones. Two copies of each core histone (H2A, H2B, H3, H4) construct a protein octamer surrounded by DNA with approximately 147 bp.

Analyzing the influence of epigenetic factors such as DNA modifications, histone modifications and chromatin remodelers on gene expression, DNA replication, and repair from the chromatin level can provide insight into the mechanism of gene regulation and cellular response to disease or treatment.

Multiple Levels of Chromatin Folding

Figure 1. Multiple Levels of Chromatin Folding. (Fyodorov D V.; et al. 2017).

With state-of-the-art technology platforms such as platforms of next-generation sequencing, mass spectrometry, and electron microscopy, Creative BioMart can offer various chromatin analysis services. Different technologies provide information on nucleic-protein interactions, chromatin accessibility, chromatin remodeling, and chromatin conformational changes.

Creative BioMart provides flexible and one-stop services for your chromatin analysis project. We can help you in any stage of experimental design, experimental implementation, and data analysis. You are free to choose the technology you prefer, but we can also provide consultation. In addition, a customized service portfolio for comprehensive and in-depth research can also be offered.

Chromatin Analysis Services at Creative BioMart

ChIP-based Service
Chromatin immunoprecipitation (ChIP) is an antibody-based, large-scale technique used to enrich and capture target protein-DNA complexes. Following the ChIP, protein-DNA interactions can be analyzed using multiple downstream applications, including ChIP-Seq, ChIP-qPCR, and ChIP-on-chip.

ATAC-Seq Service
ATAC-Seq (assay for transposase accessible chromatin with high-throughput sequencing) is a rapid and sensitive technique for studying chromatin accessibility. Finding open chromatin regions can help us gain insight into the mechanisms of gene regulation.

RIME Service
RIME (rapid immunoprecipitation mass spectrometry of endogenous proteins) utilizes immunoprecipitation of the target protein and mass spectrometry to detect endogenous interacting proteins in formaldehyde cross-linked cells. This approach is very suitable for identifying transcription cofactors and chromatin-associated proteins.

NOMe-Seq Service
NOMe-Seq (nucleosome occupancy and methylome sequencing) enables simultaneous detection of nucleosome occupancy and CpG methylation status within the same DNA molecule, allowing comprehensive detect how these epigenetic factors are interrelated.

Cryo-EM for Chromatin Remodeling Complex
The development of cryo-electron microscopy (cryo-EM) technology has accelerated the 3D structure analysis of chromatin remodeling complexes, which will help us better understand their specific biological functions and mechanisms of chromatin remodeling.

smFRET for Nucleosome Dynamics Research
Histone and/or DNA modifications can modulate the structure and dynamics of nucleosomes and consequently alter the transcriptional outcome. Single-molecule fluorescence resonance energy transfer (smFRET) technology enables monitor nucleosomes in real-time at the nanometer level to obtain relevant kinetic data.

Chromosome Conformation Capture
Chromosomal conformational capture (3C) is a technique used to analyze the form of chromosomes in the natural state of cells. The study of the structural properties and spatial organization of chromosomes is particularly important for understanding the effects of DNA modifications and altered protein binding on gene expression.

Creative BioMart fully understands the details and pitfalls of these technologies. We will work side-by-side with you to find the best design to achieve your aims. If you are interested in chromatin analysis, please don’t hesitate to contact us. We look forward to working with you.

Reference
1. Fyodorov D V.; et al. Emerging roles of linker histones in regulating chromatin structure and function. Nature Reviews Molecular Cell Biology. 2017, 19(3): 192-206.

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