Global DNA methylation analysis is often utilized as a screening tool prior to gene-specific or in-depth assays. Various methods for assessing DNA methylation at a global scale include pyrosequencing, end-specific PCR, high performance liquid chromatography (HPLC), liquid chromatography-tandem mass spectrometry (LC-MS/MS), LUminometric methylation assay (LUMA), M.SssI acceptance assay, and global DNA methylation 5mC quantification ELISA. The assay based on enzyme-linked immunosorbent assay (ELISA) enables rapid assessment of global DNA methylation status. ELISA-based assays are typically prone to high variability; thus, they are only suitable for the rough estimation of DNA methylation. Still, they are quick and easy to perform methods that serve well for the identification of large changes in global DNA methylation.

Creative BioMart provides a cost-effective and high-throughput method for the quantification of global DNA methylation. Our innovative biologists adopt a highly sensitive ELISA method to solve this problem, which is further combined with colorimetric/fluorometric technique to meet your research needs.

What Is Global DNA 5mC ELISA Quantification?

Immunoassay based on microtitration plates is known as ELISA and is a mature method that can be effectively applied to detect epigenetic modifications of DNA immobilized on plastics, using highly specific antibodies to target epigenetic markers. Briefly, the genomic DNA is denatured, fixed, and the methylated cytosines are detected through sequential incubations steps with: a) a primary antibody raised against 5mC; b) a labeled secondary antibody; and then c) colorimetric/fluorometric detection reagents.

Features of Our Global DNA 5mC ELISA Quantification

1. Cost-effective: quantitative analysis by ELISA offers cost and speed advantages. It is a cheaper, more convenient alternative than mass-spectrometry or chromatography-based techniques.
2. Tolerance of multiple sample types: it is suitable for DNA samples obtained from various species (mammals, plants, fungi, bacteria, viruses, etc). The sample types also include cultured cells, fresh and frozen tissues, paraffin-embedded tissues, as well as body fluid samples.
3. Sensitive & Accurate & High-throughput: this method requires only a small amount of input genomic DNA, exhibits linearity in the range of 1-10% of the genome, and is well correlated with the 5mC quantitative LC-MS/MS method.
4. Quality assurance: we guarantee moderate, reliable and consistent operating conditions during the experiment. Antibodies with high specificity for their target epigenetic modifications (5mC) are utilized in this assay and have no cross-reactivity with other DNA methylation variants.

Workflow of Global DNA 5mC ELISA Quantification at Creative BioMart

Figure 1. Workflow of Global DNA 5mC ELISA Quantification at Creative BioMart

Our workflow can meet your needs for small and large batches of experimental samples, and our technicians will keep you informed of the progress during the project. Working with us will greatly accelerate your research process. If you need more details about pricing and timelines, please feel free to contact us.