Histone Post-translational Modification Analysis


Histones are vulnerable to various post-translational modifications (PTMs) including but are not limited to, lysine and arginine methylation, lysine acetylation, serine and threonine phosphorylation, and lysine ubiquitination and SUMOylation. They occur at any stage in the “life cycle” of histone primarily on the flexible N-terminal tails protruding from the surface of the nucleosome as well as on the globular core region, and they are most often mediated by enzymatic activity. Histone PTMs are usually referred to as epigenetic marks in regulating gene expression by organizing the genome into active euchromatin regions, or inactive heterochromatin regions. Creative BioMart offers histone post-translational modification analysis service, which identifies and quantifies modified histone, therefore, providing essential information on the structure and dynamics of chromatin, replication and repair of DNA as well as gene expression in various pathophysiological conditions.

Traditional strategies, such as radioactive labeling, immunofluorescence and western blotting, applied in the study of histone PTMs, can be specific and relatively quantitative, but they are limited owing to the technical issues, chemical diversity, combinatorial patterns of the modifications, as well as antibody availability and specificity. In view of these limitations, mass spectrometry (MS), coupled with highly advanced sample preparation and separation techniques, is emerging as an unbiased and robust experimental platform to analyze multiple histone PTMs in a high-throughput manner. It can offer global information about individual or combinatorial PTMs patterns without a previous knowledge and locating the modification sites. Histone PTMs analysis can be completed by employing intact histones (the top-down approach), intact histone tails (the middle-down approach) or short peptide fragments (the bottom-up approach) with equal reliability. At Creative BioMart, the selection of a protocol will depend on the problem you are trying to solve and it is ultimately recommended to adopt a combination of these methods for the identification, quantification, and mechanistic characterization of a PTM histone.

Equipped with foremost technologies and PhD-level scientists with years of experience, Creative BioMart is capable of providing flexible solutions to settle various kinds of problems in analytic project of histone PTMs available for the scientific community. Our professional tools including arrays, instruments and bioinformatics together with rational design, rigorous conduction and quality assurance, will certainly accelerate your histone PTMs related research for a broad range of applications. The skilled team in Creative BioMart guides you to choose the most suitable tools and reagents for your experiments all the way from sample preparation to PTMs identification and quantification, and customizes an integrative one-stop service to achieve your goals in a high-quality, reliable and cost-effective manner.

Histone Post-translational Modification Analysis Services at Creative BioMart

Methylated Histone Quantification Assay
Histone methylation is the introduction of methyl groups to certain amino acids in a histone and it plays a critical role in the epigenetic regulation of gene transcription and may serve as a global epigenetic mark. Quantification of histone methylation has become particularly useful in studying epigenetic regulation patterns of gene expression.

Acetylated Histone Quantification Assay
Quantification of acetylated histone would provide meaningful information for further characterizing the acetylation patterns or sites, and thereby cause a better understanding about epigenetic regulation of gene activation, and exploration of drugs targeting histone acetyltransferase (HAT) or histone deacetylase (HDAC).

Phosphorylated Histone Quantification Assay
The phosphorylation of histone at Ser/Threonine is associated with chromatin condensation during mitosis and transcriptional activation of genes, DNA repair and apoptosis as well as cell growth. Detection of the phosphorylation levels in histone would afford valuable information for better understanding of the pathological process of some diseases, and for the development protein kinase-targeted drug.

Histone PTMs Identification
A huge library of histone PTMs have been described, while the correlation between the function and modification type still has not been elaborately clarified. Nevertheless, most functional data is associated with individual prominent histone PTMs that are biochemically amenable to detailed study. Abnormal histone modifications are involved in a variety of diseases. It is an essential part to identify histone PTMs pattern and sites for the understanding of its role as an epigenetic mark in cellular and physiological processes.

Advantages of Our Histone PTMs Services:
• Professional and experienced team
• Pioneering technologies and high-tech instruments
• Rational and specialized design
• Tailored PTMs histone services
• Rigorous quality assurance
• Application for various purposes

If you are obsessed with puzzles in the analysis of histone PTMs, Creative BioMart can be your stepping-stone with sophisticated service and customized support by taking advantage of both classic methods and innovative strategies in the hope of inspiring the continuous integration of proteomic and epigenetic research. If you need more detailed information on pricing and the timeline about your project, please contact us.

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