| Cat.No.: | PE-2003 |
| Product Name: | LSD1 Protein, Human, Recombinant (His & GST Tag) |
| Product Overview: | Human KDM1 (O60341-1) (Ser172-Met852) fused with the N-terminal poly histidine-tagged GST tag at the N-terminus. |
| Background: | LSD1 belongs to the flavin monoamine oxidase family. It contains 1 SWIRM domain and is a component of a RCOR/GFI/LSD1/HDAC complex. LSD1 interacts directly with GFI1 and GFI1B. LSD1 speficially removes histone H3K4me2 to H3K4me1 or H3K4me through a FAD-dependent oxidative reaction. When forming a complex with androgen receptor (and possibly other nuclear hormone receptors), LSD1 changes its substrates to H3K9me2. Thus LSD1 is considered to act as a coactivator or a corepressor. It may play a role in the repression of neuronal genes. Alone, LSD1 is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. |
| Applications: | ELISA; Western Blot , Enzyme activity assay |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | The recombinant human KDM1 /GST chimera consists of 917 amino acids and has a calculated molecular mass of 103.3 kDa. The recombinant protein migrates as an approximately 93 kDa band in SDS-PAGE under reducing conditions. |
| Purity: | > 90 % as determined by SDS-PAGE |
| Species: | Human |
| Formulation: | Lyophilized from sterile 20mM Tris, 500mM NaCl, 2mM DTT, pH 8.0, 10% gly1. |
| Accession#: | O60341-1 |
| Alternative Names: | AOF2 Protein, Human; BHC110 Protein, Human; KDM1 Protein, Human; LSD1 Protein, Human |
| Tag: | His & GST |
| Endotoxin: | < 1.0 EU per μg of the protein as determined by the LAL method |
| Expression System: | Baculovirus-Insect Cells |
| Common Name: | lysine (K)-specific demethylase 1A |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0062 | Histone Demethylase LSD1 Activity/Inhibition Assay Kit | Inquiry |
| EKIT-0126 | LSD1 Inhibitor Screening Assay Kit | Inquiry |
| EKIT-0160 | LSD1 Demethylase Activity/Inhibition Fluorometric Assay Kit | Inquiry |
| ◆ Bioactive Small Molecules | ||
| BSM-0111 | DDP-38003 dihydrochloride | Inquiry |
| BSM-0141 | GSK-LSD1 dihydrochloride | Inquiry |
| Related Gene / Proteins | |||
| LSD1 | LSD2 | LSM2 | LSM3 |
| LSM5 | LSM6 | LSP1 | |
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