| Cat.No.: | PE-2569 |
| Product Name: | Recombinant Human ARID5B protein |
| Background: | Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer. |
| Applications: | Western blot; ELISA |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | pH: 8.00; Constituents: 0.31% Glutathione, 0.79% Tris HCl |
| Alternative Names: | ARI5B_HUMAN/ARID domain-containing protein 5B/Arid5b |
| Tag: | GST |
| Amino Acid Sequence: | SGLNSRLPAGYSHSLQYLKNQTVLSPLMQPLAFHSLVMQRGIFTSPTNSQ QLYRHLAAATPVGSSYGDLLHNSIYPLAAINPQAAFPSSQLSSVHPSTKL |
| Sequence Similarities: | Belongs to the ARID5B family.Contains 1 ARID domain. |
| Expression System: | Wheat germ |
| Post Translational Modifications: | Methylation at Lys-336 prevents DNA-binding. Demethylation by PHF2 promotes recruitment of the PHF2-ARID5B complex to promoters. |
| Protein Length: | Protein fragment; 1483 to 1582 |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0001 | Human ARHGEF1 Knockout Cell Line 8bp deletion | Inquiry |
| CL-0002 | Human ARHGEF15 Knockout Cell Line | Inquiry |
| CL-0003 | Human ARHGEF11 Knockout Cell Line | Inquiry |
| CL-0004 | Human ARHGEF10L Knockout Cell Line | Inquiry |
| CL-0005 | Human ARHGEF12 Knockout Cell Line | Inquiry |
| Related Gene / Proteins | |||
| ARD1A | ARF1 | ARHGEF1 | ARHGEF10 |
| ARHGEF10L | ARHGEF11 | ARHGEF12 | ARHGEF15 |
| ARHGEF16 | ARHGEF17 | ARHGEF18 | ARHGEF19 |
| ARID1A | ARID1B | ARID2 | ARID3A |
| ARID4A | ARID5A | ARID5B | ARIH2 More > |
| AROS | ARTD10 | ||
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