| Cat.No.: | PE-3003 |
| Product Name: | EZH2-Y641F Complex |
| Description: | A five-member complex (EZH2-Y641F, AEBP2, EED, RbAp48, SUZ12) in which the catalytic subunit (EZH2-Y641F) is mutant human recombinant EZH2 with phenylalanine (F) substituted for tyrosine-641 (Y641). Acts as a histone H3 lysine-27 methyltransferase, generating trimethylated histone H3 lysine-27 (H3K27me3), an epigenetic mark essential for programmed repression of gene expression. EZH2 mutations at Y641, including Y641F, are associated with certain types of lymphoma in which they occur as heterozygotes with the wild-type allele.. While wild-type EZH2 is most efficient at monomethylation of unmethylated H3K27, the Y641F mutant has poor efficiency for this first methylation step but increased efficiency in transferring the second and third methyl groups. Thus the combined action of the mutant and wild-type enzyme produces the elevated levels of H3K27me3 observed in these lymphomas. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 333.8 kDa |
| Species: | Human |
| Formulation: | Solution of purified recombinant proteins in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol (w/v), 3 mM DTT. |
| Accession#: | NM_001203247, NM_001114176, NM_003797, NM_005610, NM_015355 |
| Tag: | Flag & His |
| Protein Length: | EZH2-Y641F 2-746, AEBP2 2-517, EED 2-441, RbAp48 2-425, SUZ12 2-739 |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0006 | GSK343 | Inquiry |
| BSM-0059 | 3-Deazaneplanocin A | Inquiry |
| BSM-0060 | 3-Deazaneplanocin A hydrochloride | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0024 | Recombinant Human EZH1 293 Cell Lysate | Inquiry |
| EL-0025 | Recombinant Human EZH2 293 Cell Lysate | Inquiry |
| Related Gene / Proteins | |||
| ezh1 | EZH2 | ||
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