| Cat.No.: | PE-3080 |
| Product Name: | Histone H1.0 |
| Description: | Full-length, untagged human recombinant histone H1.0 expressed in E. coli. H1 histones function as ‘linkers’ that bind to internucleosomal DNA (~1:1 with the histone octamer of the nucleosome core), converting nucleosomes to “chromatosomes” and promoting the formation of more compact, higher-order chromatin structure. Histone H1.0, the H1 variant most homologous to the H5 histone of avian erythrocytes, is, along with histones H1.1-H1.5, one of six H1 variants found in terminally differentiated cells. Recent work indicates that aside from playing a role in chromatin structure and function, histone H1.0 interacts with a wide array of nucleolar proteins, with specific interactions implying a role in rRNA splicing and ribosome biogenesis. In general, phosphorylation is the predominant H1 post-translational modification, notably associated with cell cycle progression, but other modifications, including lysine acetylation/methylation1 and ADP-ribosylation10, are also reported. Crosstalk occurs between H1 modifications and those on other histones and H1 variants can affect particular histone modifications, e.g. H1.3 (mouse H1d) inhibition of SET7/9 H3K4 methylation, consistent with a role for H1 in transcriptional regulation. Indeed, gene-specific transcriptional regulatory effects of H1 variants have been reported, but these remain less well studied than H1’s role in chromatin structure. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 20.9 kDa |
| Species: | Human |
| Formulation: | Solution of purified recombinant protein in 50 mM Tris/HCl pH 7.5, 750 mM NaCl, 1 mM TCEP, 10% glycerol (v/v). |
| Accession#: | NM_005318 |
| Expression System: | E. coli |
| Protein Length: | 1-194 |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Nucleosomes | ||
| NUC-0007 | Recombinant Tetranucleosomes H3.3 | Inquiry |
| NUC-0008 | Recombinant Mononucleosomes H3.3 | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0009 | Histone H4 peptide (1-21), Biotin-labeled | Inquiry |
| SP-0010 | Histone H4 peptide (1-21) | Inquiry |
| SP-0012 | Histone H3 peptide (21-44), Biotin-labeled | Inquiry |
| Related Gene / Proteins | |||
| HIC1 | HIF1A | HIF1AN | HINFP |
| HIPK1 | HIPK2 | HIRA | Histone |
| Histone H1 | Histone H2A | Histone H2B | Histone H3 |
| Histone H4 | HIV-1 reverse transcriptase | ||
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