Recombinant Nucleosome, H4K20me3 dNuc


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Cat.No.:  NUC-0073
Product Name:  Recombinant Nucleosome, H4K20me3 dNuc
Description:  Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H4 (created by a proprietary synthetic method) contains trimethyl-lysine at position 20. The nucleosome is the basic subunit of chromatin. The 601 sequence is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly.
Applications:  Nucleosome, Recombinant Human, H4K20me3 dNuc Nucleosome, Recombinant Human, H4K20me3 are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments.
Storage:  Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles.
Molecular Weight:  200,337 Da.
Species:  Human
Formulation:  Recombinant Human, H4K20me3 (2.47 μg protein weight, 50 μg DNA+protein) in 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol.
Expression System:  E. coli
Warning:  This product is for research use only. Not for use in diagnostic or therapeutic procedures.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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