| Cat.No.: | NUC-0081 |
| Product Name: | Recombinant Mononucleosomes (H2AX) |
| Description: | Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2AX, H2B, H3 and H4. Accession numbers: H2AX-P16104; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA with a 5’ biotin-TEG group. The nucleosome is the basic subunit of chromatin. H2AX is a variant of histone H2A that is phosphorylated on serine 139 by the ATM and ATR kinases in response to DNA double strand breakage. Phosphorylation of H2AX serves to recruit a number of DNA damage repair proteins, such as BRCA1 and NBS1. |
| Applications: | Mononucleosomes (H2AX), Human Recombinant Biotinylated Mononucleosomes (H2AX), Human Recombinant Biotinylated are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 202,308.2 Da. |
| Species: | Human |
| Formulation: | Purified recombinant mononucleosomes (50 µg total mass, 27.4 µg in 50.0 µl) in 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT and 20% glycerol. Concentration of nucleosomes is 4.94 µM. |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Nucleosomes | ||
| NUC-0007 | Recombinant Tetranucleosomes H3.3 | Inquiry |
| NUC-0008 | Recombinant Mononucleosomes H3.3 | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0009 | Histone H4 peptide (1-21), Biotin-labeled | Inquiry |
| SP-0010 | Histone H4 peptide (1-21) | Inquiry |
| SP-0012 | Histone H3 peptide (21-44), Biotin-labeled | Inquiry |
| Related Gene / Proteins | |||
| HIC1 | HIF1A | HIF1AN | HINFP |
| HIPK1 | HIPK2 | HIRA | Histone |
| Histone H1 | Histone H2A | Histone H2B | Histone H3 |
| Histone H4 | HIV-1 reverse transcriptase | ||
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