| Cat.No.: | NUC-0087 |
| Product Name: | Recombinant Mononucleosomes (H3.3) |
| Description: | Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.3-P84243; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. H3.3 is a histone variant, a non-allelelic replacement histone found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). H3.3-H4 associates in vivo with the HIRA chaperone complex. |
| Applications: | Mononucleosomes (H3.3), Human Recombinant Mononucleosomes (H3.3), Human Recombinant are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 200,253 Da. |
| Species: | Human |
| Formulation: | Purified recombinant mononucleosomes (100 µg total mass, protein+DNA, 54.6 µg protein by) in 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT, & 20% glycerol. |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Nucleosomes | ||
| NUC-0007 | Recombinant Tetranucleosomes H3.3 | Inquiry |
| NUC-0008 | Recombinant Mononucleosomes H3.3 | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0009 | Histone H4 peptide (1-21), Biotin-labeled | Inquiry |
| SP-0010 | Histone H4 peptide (1-21) | Inquiry |
| SP-0012 | Histone H3 peptide (21-44), Biotin-labeled | Inquiry |
| Related Gene / Proteins | |||
| HIC1 | HIF1A | HIF1AN | HINFP |
| HIPK1 | HIPK2 | HIRA | Histone |
| Histone H1 | Histone H2A | Histone H2B | Histone H3 |
| Histone H4 | HIV-1 reverse transcriptase | ||
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