| Cat.No.: | NUC-0093 |
| Product Name: | Recombinant Mononucleosomes (H3.3G34W) |
| Description: | Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) containing a methionine at position 4, expressed in E. coli. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). H3.3 is a histone variant, a non-allelelic replacement histone found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). The substitution of W for G at position 34 is associated with pediatric diffuse intrinsic pontine gliomas (DIPGs) with and diminished levels of H3K36 methylation. The 601 sequence is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group. |
| Applications: | Mononucleosomes (H3.3 G34W), Recombinant Human Biotinylated Mononucleosomes (H3.3G34W), Recombinant Human are highly purified and suitable for use as substrates in enzyme screening assays, structural studies, or effector protein binding experiments. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 200621 Da. |
| Species: | Human |
| Formulation: | Mononucleosomes (H3.3G34W), Recombinant Human (27.3 µg protein weight, 50 µg DNA+protein) in 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. |
| Tag: | Biotin |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Nucleosomes | ||
| NUC-0007 | Recombinant Tetranucleosomes H3.3 | Inquiry |
| NUC-0008 | Recombinant Mononucleosomes H3.3 | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0009 | Histone H4 peptide (1-21), Biotin-labeled | Inquiry |
| SP-0010 | Histone H4 peptide (1-21) | Inquiry |
| SP-0012 | Histone H3 peptide (21-44), Biotin-labeled | Inquiry |
| Related Gene / Proteins | |||
| HIC1 | HIF1A | HIF1AN | HINFP |
| HIPK1 | HIPK2 | HIRA | Histone |
| Histone H1 | Histone H2A | Histone H2B | Histone H3 |
| Histone H4 | HIV-1 reverse transcriptase | ||
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