Cat.No.: | PE-3343 |
Product Name: | Recombinant p53 (TP53) protein |
Product Overview: | Recombinant p53 (TP53) protein was expressed in a baculovirus expression system as the full length protein (accession number NP_000537.3) with an N-terminal FLAG tag. The molecular weight of the protein is 44.9 kDa. |
Background: | p53 (TP53) (Tumor Protein P53), also known as Phosphoprotein P53, Transformation-Related Protein 53 or Antigen NY-CO-13, is a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. It involves in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. p53 induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1, thus participating in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. It is required for apoptosis induction, which seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF, p53 also is involved in activating oxidative stress-induced necrosis, which is largely independent of transcription. |
Applications: | Suitable for use in the study of protein-protein interaction, in vitro transcription assay, binding assay. |
Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
Appearance: | Liquid |
Species: | Human |
Formulation: | 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP. |
Tag: | FLAG |
Expression System: | Baculovirus |
Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Product Types | ||
◆ Bioactive Small Molecules | ||
BSM-0182 | NSC 146109 hydrochloride | Inquiry |
BSM-0404 | Tenovin-3 | Inquiry |
◆ Research Kits | ||
EKIT-0273 | p53 Translocation Assay Kit (Cell-Based) | Inquiry |
EKIT-0274 | p53 Transcription Factor Activity Assay Kit | Inquiry |
EKIT-0420 | Human NFkB p50 Transcription Factor Assay Kit | Inquiry |
Related Gene / Proteins | |||
p50 | p53 |
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools