| Cat.No.: | NUC-0147 |
| Product Name: | Recombinant Mononucleosomes (H2A.Bbd) |
| Product Overview: | Recombinant Mononucleosomes (H2A.Bbd) - biotinylated, consist of 167 bp of 601 DNA with a 5’ biotin tag and two molecules each of histones H2A.Bbd that includes amino acids 1-115 (end) (accession number NP_001017991.1), H2B that includes amino acids 1-126 (end) (accession number NP_003509.1), H3 that includes amino acids 1-136 (end) (accession number NP_003520.1), and H4 that includes amino acids 1-103 (end) (accession number NP_003539.1). All of these histones were expressed in E. coli . The molecular weight of the histone octamer is ~107 kDa. The recombinant protein is >95% pure by SDS-PAGE. |
| Background: | In vivo, the nucleosome is the basic structural unit of chromatin. It is comprised of about 146 bp of DNA wrapped around a core of eight histones, made up of of four different types: H2A, H2B, H3 and H4. Histones are subject to posttranslational modifications, such as methylation, acetylation, phosphorylation, and mono-ubiquitination. Histone modifications influence multiple chromatin templated processes such as gene transcription, DNA repair and recombination. Besides the “major“ histones, there are some histone variants in specific regions of chromatin or in specific cell types. Histone variants are involved in multiple processes, including chromosome segregation, DNA repair, transcriptional regulation and mRNA processing. H2A.Bbd (Barr Body Deficient) is a histone H2A family member. H2A.Bbd is one of the most rapidly evolving variants, which shares 48% sequence identity with the major H2A histone. H2A.Bbd is excluded from the inactive X chromosome and is enriched in active regions of genome. H2A.Bbd is enriched at sites of DNA synthesis. H2A.Bbd nucleosomes are less stable than those bearing the major H2A histone and to organize only 118 bp of DNA, indicating that DNA in H2A.Bbd chromatin may be more accessible and, thus, more amenable to transcription. H2A.Bbd is involved in mammalian spermiogenesis. Nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery. |
| Applications: | Suitable for use as substrates in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT and 20% glycerol. |
| Tag: | Biotin |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Nucleosomes | ||
| NUC-0007 | Recombinant Tetranucleosomes H3.3 | Inquiry |
| NUC-0008 | Recombinant Mononucleosomes H3.3 | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0009 | Histone H4 peptide (1-21), Biotin-labeled | Inquiry |
| SP-0010 | Histone H4 peptide (1-21) | Inquiry |
| SP-0012 | Histone H3 peptide (21-44), Biotin-labeled | Inquiry |
| Related Gene / Proteins | |||
| HIC1 | HIF1A | HIF1AN | HINFP |
| HIPK1 | HIPK2 | HIRA | Histone |
| Histone H1 | Histone H2A | Histone H2B | Histone H3 |
| Histone H4 | HIV-1 reverse transcriptase | ||
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