| Cat.No.: | PE-3692 |
| Product Name: | Recombinant PRC2 EZH2 (A677G) complex |
| Product Overview: | Recombinant PRC2 EZH2 (A677G) complex that includes full length EZH2 with an A677G mutation complexed with full length SUZ12, EED and RbAp46/48 (accession numbers NP_001190176.1, NP_056170, NP_003788.2, NP_002884.1, and NP_005601.2, respectively) was expressed in Sf9 and contains an N-terminal FLAG-Tag at the N-terminus of EZH2. The molecular weights of expressed EZH2(A677G), SUZ12, EED and RbAp46/48 are 87 kDa, 83 kDa, 50.2 kDa, 47.8 kDa and 47.7 kDa, respectively. The recombinant protein is >90% pure by SDS-PAGE. |
| Background: | PRC2 (Polycomb Repressive Complex 2) is one of the two classes Polycomb-group, or PcG proteins (the other being PRC1) that are important epigenetic determinants of stem cell identity. They play an important role in long-term epigenetic silencing of genes during cell fate determination and differentiation. PRC2 functions as a repressor of chromatin. PRC2 is required to target recruitment to specific DNA sequences (termed Polycomb Response Elements or PREs) of genomic regions to be silenced. Once associated with chromatin, the PRC2 subunit EZH2 has histone methyltransferase activity that catalyzes the trimethylation of histone H3 at Lys27. H3K27me3 is well established as a hallmark of regions of repressed chromatin. Trimethylation of Lys27 leads to the recruitment of PRC1 through the binding of H3K27me3 by chromodomain-containing proteins in PRC1. PRC1 is responsible for long-term gene silencing after cellular differentiation. |
| Applications: | Suitable for use in the study of enzyme kinetics, inhibitor screening and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM HEPES pH 7.5, 120 mM NaCl, 0.2 mg/ml 3X FLAG peptide and 5% glycerol. |
| Tag: | FLAG |
| Expression System: | Baculovirus |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0006 | GSK343 | Inquiry |
| BSM-0027 | UNC0321 (trifluoroacetate salt) | Inquiry |
| BSM-0047 | SGC707 | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0024 | Recombinant Human EZH1 293 Cell Lysate | Inquiry |
| EL-0025 | Recombinant Human EZH2 293 Cell Lysate | Inquiry |
| Related Gene / Proteins | |||
| ezh1 | EZH2 | Pr-SET7 | PRC1 |
| PRC2 | PRDM1 | PRDM10 | PRDM11 |
| PRDM12 | PRDM13 | PRDM14 | PRDM15 |
| PRDM16 | PRDM17 | PRDM2 | PRDM3 |
| PRDM4 | PRDM5 | PRDM6 | PRDM7 More > |
| PRDM8 | PRDM9 | PREP1 | PRIM2 |
| PRKAA2 | PRKCA | PRKCB | PRKCE |
| PRKDC | PRKRIP1 | PRMT | prmt1 |
| PRMT2 | PRMT3 | PRMT5 | prmt6 |
| PRMT7 | PRMT8 | PRMT9 | Progerin |
| Protamine 2 | Prox1 | PRPF31 | PRPF40A |
| PRSS12 | PRSS55 | PRTFDC1 | |
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