| Cat.No.: | PE-3715 |
| Product Name: | Recombinant EHMT2 (G9A)-SET (913-1193) protein |
| Product Overview: | The peptide corresponding to amino acids 913 - 1193 of the EHMT2-SET (G9a) protein (accession number NP_006700.3) was expressed in E. coli. The peptide contains the SET domain of G9a that is responsible for methyltransferase activity. Recombinant G9a-SET contains N-terminal GST-Tag with an observed molecular weight of 58.7 kDa. |
| Background: | EHMT2-SET (G9a), also known as Euchromatic Histone-lysine N-methyltransferase 2 (EHMT2), is a histone methyltransferase that specifically mono- and dimethylates Lys9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by initiating recruitment of HP1 proteins to methylated histones. EHMT2-SET (G9a) also mediates monomethylation of Lys56 of histone H3 (H3K56me1) in G1 phase and regulates DNA replication by promoting the interaction between histone H3 and PCNA. G9a also weakly methylates Lys27 of histone H3 (H3K27me). In addition, G9a is required for DNA methylation. However, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. G9a is most likely targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. G9a may also methylate histone H1. In addition to histone methyltransferase activity, G9a also methylates non-histone proteins. It has been shown to mediate dimethylation of Lys373 of p53 and also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. |
| Applications: | Suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM Tris-HCl pH 8.0, 300 mM NaCl and 5% glycerol. |
| Tag: | GST |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0025 | BIX01294 (hydrochloride hydrate) | Inquiry |
| BSM-0026 | UNC0224 | Inquiry |
| BSM-0027 | UNC0321 (trifluoroacetate salt) | Inquiry |
| ◆ Antibodies | ||
| EAb-0030 | SETD8 Polyclonal Antibody | Inquiry |
| EAb-0031 | SETDB1 Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| EHD2 | EHMT1 | EHMT2 | SENP3 |
| SENP8 | SET | SET1 | SET2 |
| SET7 | SET8 | SET9 | SETBP1 |
| SETD1A | SETD1B | SETD2 | SETD3 |
| SETD5 | SETD6 | SETD7 | SETD8 More > |
| SETD9 | SetDB1 | SETDB2 | SETMAR |
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools
