| Cat.No.: | PE-3826 |
| Product Name: | Histone H2B (human), (recombinant) |
| Background: | The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The core histones form an octamer which is made up of one H3-H4 tetramer and two H2A-H2B dimers. The C-terminal domains of the core histones have very similar conformations that are important for nucleosome assembly. The N-terminal tails are less structured which is thought to allow for dynamic changes in the accessibility of the underlying genome. The N-terminal tail of core histones undergo different posttranscriptional modifications including acetylation, phosphorylation, methylation and ubiquitination. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. Histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. histone H2B is phosphorylated in apoptotic cells and this event occurs around the time of nucleosomal DNA fragmentation. Phosphorylation of both histone H2A.X and H2B is dependant on caspase activation and therefore may be linked to caspase-induced signalling pathways. |
| Applications: | Western blot control; Kinase assay |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Molecular Weight: | ~15kDa |
| Purity: | ≥95% (SDS-PAGE; Western blot) |
| Formulation: | 1x Dulbecco's PBS |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Nucleosomes | ||
| NUC-0007 | Recombinant Tetranucleosomes H3.3 | Inquiry |
| NUC-0008 | Recombinant Mononucleosomes H3.3 | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0009 | Histone H4 peptide (1-21), Biotin-labeled | Inquiry |
| SP-0010 | Histone H4 peptide (1-21) | Inquiry |
| SP-0012 | Histone H3 peptide (21-44), Biotin-labeled | Inquiry |
| Related Gene / Proteins | |||
| HIC1 | HIF1A | HIF1AN | HINFP |
| HIPK1 | HIPK2 | HIRA | Histone |
| Histone H1 | Histone H2A | Histone H2B | Histone H3 |
| Histone H4 | HIV-1 reverse transcriptase | ||
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