Cat.No.: | EKIT-0228 |
Product Name: | DNMT1 Chemiluminescent Assay Kit |
Size: | 96 reactions |
Description: | The DNMT1 Assay Kit is designed to measure DNMT1 activity for screening and profiling applications. The key to the DNM1 Assay Kit is a highly specific antibody that recognizes 5-methylcytosine on the substrate. With this kit, only three simple steps on a microtiter plate are required for DNMT1 detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT1 for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. |
Kit Components: | DNMT1: 2 x 10 µg; -80°C400 µM S-adenosylmethionine: 2 x 250 µl; -80°C Anti-5-methylcytosine antibody: 25 µl; -80°C Secondary HRP-labeled antibody 1: 10 µl; -80°C 4x DNMT assay buffer 1: 5 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate A: 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C Strip plate precoated with DNMT1 substrate: 1; +4°C |
Target Species: | Mouse, Human; Mammals |
Warning: | For research use only, not for diagnostic use. |
Product Types | ||
◆ Bioactive Small Molecules | ||
BSM-0008 | Vinorelbine Tartrate | Inquiry |
◆ Extracts & Lysates | ||
EL-0027 | Recombinant Human DNMT3A 293 Cell Lysate | Inquiry |
EL-0028 | Recombinant Human DNMT3B 293 Cell Lysate | Inquiry |
EL-0029 | Recombinant Human DNMT3L 293 Cell Lysate | Inquiry |
EL-0030 | Recombinant Human DNMT3L 293 Cell Lysate | Inquiry |
Related Gene / Proteins | |||
DNA alkyltransferase | DNAJC2 | DNAS1L1 | DNASE1L3 |
DNMT | DNMT1 | DNMT2 | DNMT3A |
DNMT3B | DNMT3L | DNMT4 |
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