BRD4 (BD1) Inhibitor Screening Assay Kit


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Cat.No.:  EKIT-0250
Product Name:  BRD4 (BD1) Inhibitor Screening Assay Kit
Size:  384 reactions
Description:  The BRD4 (BD1) Inhibitor Screening Assay Kit is designed to measure the inhibition of BRD4 bromodomain 1 (BD1) from binding to its substrate. The key to the BRD4 (BD1) Inhibitor Screening Assay Kit is the highly specific binding of the BRD4 bromodomain 1 to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRD4 bromodomain 1 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Kit Components:  BRD4, BD1, GST-tag: 20 µg; -80°CBET Bromodomain Ligand (BRD4-BD1): 400 µl; -80°CNon-acetylated Ligand: 200 µl; -80°C3x BRD Homogeneous Assay Buffer 1: 4 ml; -20°C3x BRD Homogeneous Detection Buffer 1: 3 ml; -20°C
Warning:  For research use only, not for diagnostic use.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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