| Cat.No.: | EKIT-0311 |
| Product Name: | m6A RNA Methylation Assay Kit (Colorimetric) |
| Assay Type: | Quantitative |
| Description: | The m6A RNA Methylation Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify methylated N6-methyladenosine (m6A) in RNA. It is suitable for a direct detection of m6A RNA methylation status using total RNA isolated from any species such as mammals, plants, fungi, bacteria, and viruses.The amount of RNA for each assay can be 100 ng - 300 ng. For optimal quantification, the input RNA amount should be 200 ng, as the abundance of m6A is generally less tha 0.1% of total RNA. |
| Background: | N6-methyladenosine (m6A) is the most common and abundant modification in RNA molecules present in eukaryotes. The m6A modification is catalyzed by a methyltransferase complex METTL3 and removed by the recently discovered m6A RNA demethylases FTO and ALKBH5, which catalyze m6A demethylation in an α-ketoglutarate (α-KG)- and Fe2+-dependent manner. It was shown that METTL3, FTO, and ALKBH5 play important roles in many biological processes, ranging from development and metabolism to fertility. m6A accounts for more than 80% of all RNA base methylations and exists in various species. m6A is mainly distributed in mRNA and also occurs in non-coding RNA such as tRNA, rRNA, and snRNA. The relative abundance of m6A in mRNA transcripts has been shown to affect RNA metabolism processes such as splicing, nuclear export, translation ability and stability, and RNA transcription. Abnormal m6A methylation levels induced by defects in m6A RNA methylase and demethylase could lead to dysfunction of RNA and diseases. The dynamic and reversible chemical m6A modification in RNA may also serve as a novel epigenetic marker of profound biological significance. |
| Range: | 0.01 ng/ml - 0.5 ng/ml |
| Sensitivity: | 0.01 ng |
| Detection Method: | Colorimetric |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Antibodies | ||
| EAb-0260 | N6-Methyladenosine Monoclonal Antibody (17-3-4-1) | Inquiry |
| EAb-1987 | N6-Methyladenosine (m6A) Monoclonal Antibody | Inquiry |
| EAb-1988 | N6-Methyladenosine (m6A) Polyclonal Antibody | Inquiry |
| ◆ Research Kits | ||
| EKIT-0311 | m6A RNA Methylation Assay Kit (Colorimetric) | Inquiry |
| Related Gene / Proteins | |||
| m6A | |||
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