| Cat.No.: | EKIT-0327 |
| Product Name: | Histone H3 (pan-methyl K79) Quantification Kit (Fluorometric) |
| Description: | Methylation of histone H3 at lysine 79 is catalyzed by Dot1 in mammalian cells. Intergenic H3 (mono-methyl K79) is a mark of enhancer regions and may provide unique binding surfaces along the chromatin fibre to stabilize interactions between enhancers and their target promoters. H3 (di-methyl K79) and H3 (tri-methyl K79) are also widespread histone modifications and are associated with transcriptionally active genes. Increased global H3 (methyl K79) is also found to be involved in some pathological processes such as leukemogenesis in humans.Histone H3 (pan-methyl K79) Quantification Kit (Fluorometric) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3K79 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells. |
| Background: | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Range: | 5 ng/well - 2000 ng/well |
| Sensitivity: | 0.4 ng/well |
| Detection Method: | Fluorescent |
| Target Species: | Mouse, Human; Mammals |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0001 | Acetyl Histone H3-K14 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0002 | Acetyl Histone H3-K14 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0003 | Acetyl Histone H3-K18 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0004 | Acetyl Histone H3-K18 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0005 | Acetyl Histone H3-K23 Quantification Kit (Colorimetric) | Inquiry |
| Related Gene / Proteins | |||
| H3K10 | H3K115 | H3K122 | H3K123 |
| H3K14 | H3K18 | H3K23 | H3K27 |
| H3K36 | H3K37 | H3K4 | H3K5 |
| H3K56 | H3K64 | H3K79 | H3K80 |
| H3K9 | H3R128 | H3R17 | H3R2 More > |
| H3R26 | H3R8 | H3R9 | H3S10 |
| H3S28 | H3S31 | H3S32 | H3T11 |
| H3T118 | H3T3 | H3T32 | H3T41 |
| H3T45 | H3T6 | H3T80 | H3Y41 |
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