Histone H3 (di-methyl K79) Quantification Kit (Colorimetric)


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Cat.No.:  EKIT-0359
Product Name:  Histone H3 (di-methyl K79) Quantification Kit (Colorimetric)
Description:  Methylation of histone H3 at lysine 79 is catalyzed by Dot1 in mammalian cells. H3 (di-methyl K79) is a widespread histone modification and is associated with transcriptionally active genes. Histone H3 (di-methyl K79) Quantification Kit (Colorimetric) allows the user to specifically measure global di-methylation of histone H3K79 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
Background:  Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Range:  20 ng/well - 5000 ng/well
Sensitivity:  2 ng/well
Detection Method:  Colorimetric
Target Species:  Mouse, Human; Mammals
Warning:  For research use only, not for diagnostic use.

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