SMYD3 Homogeneous Assay Kit


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Cat.No.:  EKIT-0476
Product Name:  SMYD3 Homogeneous Assay Kit
Size:  384 reactions
Description:  The SMYD3 Homogeneous Assay Kit is designed to measure SMYD3 activity for screening and profiling applications. SMYD3 is a histone methyltransferase that promotes diand trimethylation of lysine4 of histone H3. It also exhibits methylation activity toward a lysine residue of MAPK/ERK Kinase Kinase 2 (MAP3K2). The key to the SMYD3 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing SMYD3 enzyme is incubated with the substrate for three hours. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Applications:  For studying enzyme kinetics and HTS applications.
Kit Components:  The SMYD3 Homogeneous Assay Kit comes in a convenient AlphaLISA format, with GST-tagged SMYD3 substrate, primary antibody, methylation assay buffer, and purified SMYD3 for 384 enzyme reactions.
Warning:  For research use only, not for diagnostic use.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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