SetDB1 Chemiluminescent Assay Kit


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Cat.No.:  EKIT-0479
Product Name:  SetDB1 Chemiluminescent Assay Kit
Size:  96 reactions
Description:  The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1 activity for screening and profiling applications. The key to the SetDB1 Direct Activity Assay Kit is a highly specific antibody that recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Applications:  For studying enzyme kinetics and HTS applications.
Kit Components:  Kit comes in a convenient format, with wells precoated with histone H3 peptide substrate, antibody against methylated lysine residue of Histone H3, secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase buffer, and purified enzyme
Warning:  For research use only, not for diagnostic use.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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