PRMT5 Chemiluminescent Assay Kit

Cat.No.:	EKIT-0489
Product Name:	PRMT5 Chemiluminescent Assay Kit
Size:	96 reactions
Description:	The PRMT5 Direct Activity Chemiluminescent Assay Kit is designed to measure PRMT5 activity for screening and profiling applications. The key to the PRMT5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Background:	PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3.
Applications:	For studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Kit Components:	Kit comes in a convenient format, with a 96-well plate precoated with histone H4 peptide substrate, reagents, and purified PRMT5 enzyme for 100 enzyme reactions.
Warning:	For research use only, not for diagnostic use.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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