Cat.No.: | EKIT-0500 |
Product Name: | METTL3/METTL14 Complex Chemiluminescent Assay Kit |
Size: | 96 Reactions |
Description: | The METTL3/METTL14 Complex Chemiluminescent Assay Kit is designed to measure the METTL3/METTL14 complex activity for screening and profiling applications. The METTL3/METTL14 Complex Chemiluminescent Assay Kit comes in a convenient format, with 96-well plate precoated with a specific substrate, the antibody against methylated adenosine, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified METTL3/METTL14 complex enzyme for 96 enzyme reactions. The key to the METTL3/METTL14 Complex Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes N6methylated adenosine. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. |
Applications: | For studying enzyme kinetics and HTS applications. |
Kit Components: | The METTL3/METTL14 Complex Chemiluminescent Assay Kit comes in a convenient format, with 96-well plate precoated with a specific substrate, the antibody against methylated adenosine, a secondary HRP-labeled antibody, S-adenosylmethionine, methylt |
Warning: | For research use only, not for diagnostic use. |
Product Types | ||
◆ Extracts & Lysates | ||
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EL-0111 | Recombinant Human MEF2B Cell Lysate | Inquiry |
◆ Antibodies | ||
EAb-0067 | MeCP2 Polyclonal Antibody | Inquiry |
◆ Cell Lines | ||
CL-0109 | Human MECP2 Knockout Cell Line 1bp insertion | Inquiry |
CL-0110 | Human MEN1 Knockout Cell Line 1bp insertion | Inquiry |
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