Recombinant Human PRKCB 293 Cell Lysate


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  • Gene Information
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Cat.No.:  EL-0102
Product Name:  Recombinant Human PRKCB 293 Cell Lysate
Description:  Antigen standard for protein kinase C, beta (PRKCB), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Species:  Human
Source:  HEK 293 Cells
Components:  This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Storage:  Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C.
Warning:  For research use only, not for diagnostic or therapeutic use.
Gene ID NCBI:  5579
Official Symbol:  PRKCB
Official Full Name:  protein kinase C, beta
Synonyms:  PRKCB; protein kinase C, beta; PKCB, PRKCB1, PRKCB2, protein kinase C, beta 1; protein kinase C beta type; PKC-B; protein kinase C, beta 1 polypeptide; PKCB; PRKCB1; PRKCB2; PKC-beta; MGC41878;
mRNA Refseq:  NM_002738
Protein Refseq:  NP_002729
MIM:  176970
UniProt ID:  P05771
Chromosome Location:  16p12
Function:  ATP binding; androgen receptor binding; chromatin binding; histone binding; histone kinase activity (H3-T6 specific); ligand-dependent nuclear receptor transcription coactivator activity; metal ion binding; nucleotide binding; protein binding; protein kinase C activity; protein kinase C binding; zinc ion binding;

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