Recombinant Human TRDMT1 Cell Lysate


  • Specification
  • Gene Information
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Cat.No.:  EL-0121
Product Name:  Recombinant Human TRDMT1 Cell Lysate
Product Overview:  Human TRDMT1 / DNMT2 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.
Species:  Human
Source:  Baculovirus-Insect Cells
Lysis Buffer:  Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Recommended Usage:  1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage:  Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Warning:  For research use only, not for diagnostic or therapeutic use.
Gene ID NCBI:  1787
Official Symbol:  TRDMT1
Official Full Name:  tRNA aspartic acid methyltransferase 1
Synonyms:  TRDMT1; tRNA aspartic acid methyltransferase 1; DNA (cytosine 5 ) methyltransferase 2 , DNMT2; tRNA (cytosine(38)-C(5))-methyltransferase; RNMT1; DNA methyltransferase-2; DNA MTase homolog HsaIIP; tRNA (cytosine-5-)-methyltransferase; DNA (cytosine-5)-methyltransferase-like protein 2; DMNT2; DNMT2; PUMET; MHSAIIP;
mRNA Refseq:  NM_004412
Protein Refseq:  NP_004403
MIM:  602478
UniProt ID:  O14717
Chromosome Location:  10p15.1
Function:  DNA (cytosine-5-)-methyltransferase activity; DNA binding; RNA binding; methyltransferase activity; transferase activity;

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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