Recombinant Human SMYD1 293 Cell Lysate


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Cat.No.:  EL-0124
Product Name:  Recombinant Human SMYD1 293 Cell Lysate
Description:  Antigen standard for SET and MYND domain containing 1 (SMYD1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Species:  Human
Source:  HEK 293 Cells
Components:  This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Storage:  Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C.
Warning:  For research use only, not for diagnostic or therapeutic use.
Gene ID NCBI:  150572
Official Symbol:  SMYD1
Official Full Name:  SET and MYND domain containing 1
Synonyms:  SMYD1; SET and MYND domain containing 1; SET and MYND domain-containing protein 1; BOP; KMT3D; ZMYND22; CD8 beta opposite; zinc finger, MYND domain containing 18; ZMYND18;
mRNA Refseq:  NM_198274
Protein Refseq:  NP_938015
MIM:  606846
UniProt ID:  Q8NB12
Chromosome Location:  2p11.1
Function:  DNA binding; metal ion binding; transcription corepressor activity; zinc ion binding;

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